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Identification des bases génétiques associées à la variation naturelle des interactions plante-plante chez Arabidopsis thaliana

Abstract : Biotic interactions are crucial in the response of plant communities to environmental changes. Among these interactions, plant-plant interactions play an important role in the structure, diversity and dynamics of plant communities. Although it is widely accepted that the identification of genes associated with plant-plant interactions is an important step in predicting and understanding the adaptive dynamics of plant communities, studies on the identification of genetic variants associated with natural variation in plant-plant interactions remain scarce. The main objective of this thesis was to characterize and identify the genetic bases associated with natural variation of competitive response in a local population (TOU-A) of the model species Arabidopsis thaliana interacting with different species. In a first chapter, with a resurrection approach coupled with GWA mapping and a temporal genetic differentiation analysis, I was able to show that the TOU-A population was adapted for the identification of associated adaptive genetic bases underlying monospecific plant-plant interactions. In the second chapter, in order to take into account the complexity of plant-plant interactions observed in nature, I was interested in characterizing the genetic architecture of the competitive response of A. thaliana in different contexts of mono- and multispecific interactions. I was able to highlight biotic specialization of some accessions in response to certain assemblages, and this, despite observing a similar response of the entire population in response to different interaction treatments. Through an approach of GWA mapping, I was able to highlight that the QTLs of competitive response of A. thaliana were mostly different between the 12 interaction treatments. I have also been able to show that biological processes differ between monoand multi-specific conditions and, more specifically, that receptor like-kinase play a major role in plant-plant interactions. In a third chapter, I sought to functionally validate a gene underlying a QTL associated with the competitive response of A. thaliana to Poa annua. By phenotyping mutant lines and complemented lines, I was able to identify that the PERK13/RHS10 gene was the causative gene underlying this QTL. This functional validation allowed me to characterize PERK13/RHS10 as a positive regulator of a competition avoidance strategy in response to P. annua but also to wheat (Triticum aestivum). This work is in line with interdisciplinary approaches, which intends to improve our understanding of the genetic determinants that underlie the adaptive natural variation of biotic interactions. Keywords: ecological genomics, plant-plant interactions, local population, natural variation, genetic bases, GWA mapping, functional validation, Arabidopsis thaliana.
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Submitted on : Thursday, February 20, 2020 - 10:16:49 AM
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Cyril Libourel. Identification des bases génétiques associées à la variation naturelle des interactions plante-plante chez Arabidopsis thaliana. Sciences du Vivant [q-bio]. Université Paul Sabatier (Toulouse 3), 2019. Français. ⟨tel-02485393v1⟩



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