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2P optogenetics : simulation and modeling for optimized thermal dissipation and current integration

Abstract : Over the past fifteen years, optogenetics has revolutionized neuroscience research by enabling control of neuronal circuits. The recent development of several illumination approaches, combined with new photosensitive proteins, opsins, have paved the way to neuronal control with the single-cell precision. The new ambition to use these approaches in order to activate tens, hundreds, thousands of cells in vivo has raised many questions, in particular concerning the possible photoinduced damages and the optimization of the choice of the illumination / opsin couple. During my PhD, I developed an experimentally verified simulation that calculates, under all actual illumination protocols, what will be the temperature rise in the brain tissue due to the absorption of light. In parallel, I modeled, from electrophysiology recordings, the intracellular currents observed during these photostimulations, for three different opsins, allowing me to simulate them. These models will allow the researchers to optimize their illumination protocols to keep heating as low as possible in the sample, while helping to generate optimized photocurrent dynamics according to experimental requirements.
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Alexis Picot. 2P optogenetics : simulation and modeling for optimized thermal dissipation and current integration. Optics [physics.optics]. Université Sorbonne Paris Cité, 2018. English. ⟨NNT : 2018USPCB227⟩. ⟨tel-02469061⟩

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