Skip to Main content Skip to Navigation
Theses

Subthreshold Ca²+-dependent modulation of vesicle release dynamics and docking site occupancy at single central synapses

Abstract : In several neurons of the CNS, subthreshold somatodendritic activity can spread passively into the axon and transiently enhance spontaneous and spike-evoked synaptic transmission in a Ca2+-dependent and graded manner. Available evidence about the underlying mechanism of this type of synaptic plasticity, called “analog” or “analog to digital” facilitation (ADF), remains largely incomplete for the majority of central synapses, mainly due to the experimental inaccessibility to the small presynaptic boutons. Here we use both Ca2+ photolysis and imaging at individual presynaptic terminals of the rat cerebellar molecular layer interneurons (MLIs), combined with whole-cell paired recordings from synaptically connected MLIs, to report a novel subthreshold Ca2+-dependent mechanism for ADF whereby the fraction and the kinetics of the pool of vesicles available for immediate release, the readily releasable pool (RRP), are modulated by changing the docking site occupancy probability in single synaptic contacts. Our results add a new dimension in the understanding of how subthreshold activity modulates information flow in neuronal circuits.
Document type :
Theses
Complete list of metadatas

Cited literature [96 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02469015
Contributor : Abes Star :  Contact
Submitted on : Thursday, February 6, 2020 - 12:12:08 PM
Last modification on : Thursday, June 4, 2020 - 6:04:57 PM
Long-term archiving on: : Thursday, May 7, 2020 - 2:32:18 PM

File

va2_Blanchard-Tapia_Kris.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02469015, version 1

Citation

Kris Blanchard Tapia. Subthreshold Ca²+-dependent modulation of vesicle release dynamics and docking site occupancy at single central synapses. Neurons and Cognition [q-bio.NC]. Université Sorbonne Paris Cité; Universidad de Chile, 2018. English. ⟨NNT : 2018USPCB206⟩. ⟨tel-02469015⟩

Share

Metrics

Record views

66

Files downloads

20