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Étude des processus de biominéralisation de la nacre chez l'ormeau européen haliotis tuberculata

Abstract : The mother-of-pearl found in the shell of certain mollusks is a fascinating material with remarkable physical (mechanical and optical) properties. This biological material of organic and mineral composite nature presents a complex architecture whose structure and mechanisms of formation are not yet perfectly understood. In this work, taking as a model study the European abalone Haliotis tuberculata, we sought to contribute to the knowledge around this material by focusing our study on three aspects in particular: i) the ultrastructural study of the phase mineral and more particularly disordered mineral environments, ii) the study of the organo-minerale interface in the adult nacre in formation as well as in the larval shell and iii) the study of the first biominerals deposited in the larval shell at very young stages of development. To carry out this study, we used a coupling of advanced spectroscopic techniques (Solid-State Nuclear Magnetic Resonance, Scanning transmission X-ray microscopy and Electron energy loss spectroscopy) coupled to advanced electron microscopy techniques (High-Resolution Transmission Electronic Microscopies and Field Emission Gun Scanning Electronic Microscopy) and preparation of sample (Focused Ion beam) and molecular biology (immunohistochemistry, Correlative Light and Electronic Microscopy).
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Widad Ajili. Étude des processus de biominéralisation de la nacre chez l'ormeau européen haliotis tuberculata. Chimie inorganique. Sorbonne Université, 2018. Français. ⟨NNT : 2018SORUS160⟩. ⟨tel-02466424⟩

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