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Apport du séquençage haut débit dans l'analyse bioinformatique du génome du virus de l'hépatite C

Abstract : High-throughput sequencing has been used in this work to reconstruct with adapted methods the whole genomeof the hepatitis C virus (HCV) particularly for accurately typing the virus. Thus, we managed to detect in a studya recombinant form of HCV circulating within a patient. We typed and detected in another study resistancemutations of several HCV strains of different genotypes. Finally, a last study based on this approach enabled touncover a HCV strain belonging to a new subtype. High-throughput sequencing has also been used in this workto detect multiple infections and analyze viral evolution with targeted HCV genes and non-specific methods for2 HCV patients under treatment. This retrospective study enabled to define the composition of each temporalsample, assess their nucleotide diversity, investigate viral population genetic structure and temporal evolutionand date secondary infections. Results of this analysis support the hypothesis of onset mechanism of treatmentresistance (selective sweeps).
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Alban Caporossi. Apport du séquençage haut débit dans l'analyse bioinformatique du génome du virus de l'hépatite C. Biotechnologies. Université Grenoble Alpes, 2019. Français. ⟨NNT : 2019GREAS021⟩. ⟨tel-02460561⟩

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