Etude des isoformes du gène PGC-1a dans le développement musculaire chez le bovin

Abstract : The transcriptional co-activator PGC-1α (PPARGC1A) has been reported to play a key role in adaptive thermogenesis and to influence muscle homeostasis and growth in mouse and human. PGC-1α has a complex structure with multiple protein domains whose gene is controlled by two promoters and is subject to alternative splicing events. In cattle, very little is currently known about PGC-1α, despite its implication in growth and milk characteristics revealrd by SNP study. So, the aim of our study was to investigate the presence and the structure of bovine PGC-1α alternative transcripts. We found different transcripts, two full-length isoforms named PGC-1α-a and PGC-1α-b, and two truncated forms, NT-PGC-1α and PGC-1α4. In basal conditions, our results showed that the truncated forms are the most expressed in bovine muscle. In addition, the transcripts derived from the proximal promoter are predominant, suggesting that NT-PGC-1 would be the main form. Finally, we showed that the overexpression of either fulllength or truncated isoforms of bovine PGC-1 enhances myoblasts differentiation. The multiplicity of isoforms resulting from PGC-1α as well as their implication in myogenesismakes PGC-1α as a gene of interest for the study of the muscular phenotypic variability found in different cattle breeds. In addition, PGC-1 transctipts appear to be a strong modulators of muscle mass. So the bovine PGC-1a isoforms could be used to engineer future breeds with higher muscularity.
Document type :
Theses
Complete list of metadatas

Cited literature [178 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02440685
Contributor : Abes Star <>
Submitted on : Wednesday, January 15, 2020 - 12:11:07 PM
Last modification on : Thursday, January 16, 2020 - 1:30:59 AM

File

2018LIMO0057.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02440685, version 1

Collections

Citation

Jessica Bamba Funck. Etude des isoformes du gène PGC-1a dans le développement musculaire chez le bovin. Médecine humaine et pathologie. Université de Limoges, 2018. Français. ⟨NNT : 2018LIMO0057⟩. ⟨tel-02440685⟩

Share

Metrics

Record views

258

Files downloads

9