I. V. !-figure, ) les SIgA interagiraient avec un(des) r?cepteur(s) inconnu(s) (disque gris avec un point dÕinterrogation). (2) La fixation des SIgA induirait le recrutement de complexes prot?iques compos?s de CD71, SCAMP3 et BCAP31, d?j? form?s ? lÕ?tat basal. (3) HM13 serait pr?sent au sein des complexes CD71-SCAMP3-BCAP31 ? lÕ?tat basal et/ou recrut? de mani?re d?pendante de CD71 pour former le Ç cÏur È du complexe de fixation des SIgA. (4) LÕoligom?risation de plusieurs complexes serait ensuite initi?e par SCAMP3, avec la participation potentielle des autres partenaires et des SIgA dans la stabilisation des oligom?res. Ces agr?gats de taille importante seraient ensuite endocyt?s selon un m?canisme d?pendant du(des) r?cepteur(s) inconnu(s) et/ou dÕautre(s) membre(s) de ces larges agr?gats afin dÕ?tre r?tro-transport?s ? travers lÕIEC, Repr?sentation hypoth?tique des complexes prot?iques form?s par les pIgA1 M et les SIgA ? la surface des cellules Caco-2 TC7. Dans les cellules contr?les, vol.1

, LÕoligom?risation de plusieurs complexes serait ensuite initi?e par SCAMP3, avec la participation potentielle des autres partenaires et des pIgA1 M dans la stabilisation des oligom?res. Ces agr?gats membranaires de taille importante seraient ensuite endocyt?s selon un m?canisme d?pendant de CD71 et/ou dÕautre(s) partenaire(s) inconnu(s) du complexe prot?ique, Dans les cellules contr?les (en bas au centre), (1Õ) Les pIgA1 M interagissent directement avec CD71, pr?sent sous forme de complexes avec SCAMP3 et BCAP31 ? lÕ?tat basal. (2Õ) HM13, d?j? pr?sent au sein des complexes CD71-SCAMP3-BCAP31 ? lÕ?tat basal et/ou recrut? de mani?re d?pendante de CD71 viendrait former le Ç cÏur È du complexe de fixation des pIgA1 M . (3Õ)

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%. and -. %#-%)&quot;*#/0123#2456708591, Les cellules Caco-2 TC7 ont ?t? lys?es en tampon de lyse contenant 1% de Brij O10 ou en tampon RIPA et 20 #g de prot?ines ont ?t? mis ? migrer en SDS-PAGE en conditions d?naturantes et r?ductrices (5% $-ME). La r?v?lation des membranes ? lÕaide dÕun anticorps sp?cifique de SCAMP3 indique la pr?sence de monom?res (~35 kDa) et de dim?res (60 Ð 70 kDa) absents, 3# %)(*#<=<# Figure suppl?mentaire 2 : SCAMP3 semble former des dim?res stables dans les cellules Caco-2 TC7

, Les IgA sont produites dans le chorion sous forme majoritairement polym?rique (pIgA) et s?cr?t?es dans la lumi?re des muqueuses, Parmi les cinq isotypes dÕanticorps pr?sents chez lÕHomme, les immunoglobulines A (IgA) pr?dominent dans les muqueuses

, B-Cell Receptor-Associated Protein 31 (BCAP31) et Histocompatibility minor 13 (HM13) comme membres du complexe SIgA-CD71. En nous appuyant ? nouveau sur la technologie CRIPSR-Cas9, nous avons montr? quÕaucun de ses partenaires nÕinteragit directement avec les SIgA. N?anmoins, nos r?sultats sugg?rent que SCAMP3 est n?cessaire ? lÕoligom?risation de surface des complexes de fixation des SIgA. Enfin, lÕinternalisation des SIgA n'est pas alt?r?e par lÕabsence de chacun des partenaires du complexe, sugg?rant que leur r?le advient durant le transport trans-?pith?lial des SIgA. LÕensemble de nos travaux montre que les SIgA interagissent avec l'?pith?lium intestinal via un complexe prot?ique compos? d'au moins cinq membres : CD71, SCAMP3, BCAP31, HM13 et le(s) r?cepteur(s) inconnu(s) aux SIgA. Ces r?sultats compl?tent les travaux ant?rieurs sur le r?le physiopathologique des SIgA dans la maladie cÏliaque et contribuent ?, Une fonction essentielle des SIgA intestinales est de maintenir microorganismes et antig?nes alimentaires dans la lumi?re des muqueuses afin dÕemp?cher leur p?n?tration dans l'organisme. N?anmoins, la transcytose inverse des SIgA coupl?es ? des bact?ries a ?t? d?crite dans les plaques de Peyer o? elle participe ? la g?n?ration de la r?ponse immune contre ces bact?ries. En outre, les travaux pass?s du laboratoire ont sugg?r? que le r?cepteur de la transferrine (CD71), vol.3