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Effet de l'encombrement des protéines sur la diffusion des lipides et des protéines membranaires

Abstract : Lateral diffusion of lipids and transmembrane proteins is essential for biological functions. In the cellular context, the surface fraction of membrane proteins is high, reaching approximately 50 to 70% depending on the membrane type. Therefore, diffusion occurs in a very crowded environment. The aim of this work is to study in vitro the effect of protein crowding on their own diffusion and on those of the surrounding lipids. So far, lateral diffusion measurements generally have been carried out at low protein density, and the effect of proteins crowding has not been much studied experimentally. We used a single particle tracking (SPT) method to track the trajectories of the Bacterorhodopsin (BR) proton pump and of lipids labeled with quantum dots at the bottom of giant unilamellar vesicles (GUVs) as a function of the total surface fraction (Ф) of BR reconstituted in 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) membrane.
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  • HAL Id : tel-02420527, version 1

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Kodjo Mawoussi. Effet de l'encombrement des protéines sur la diffusion des lipides et des protéines membranaires. Biophysique. Université Pierre et Marie Curie - Paris VI, 2017. Français. ⟨NNT : 2017PA066541⟩. ⟨tel-02420527⟩

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