Skip to Main content Skip to Navigation
Theses

Sen1-mediated RNAPIII transcription termination controls the positioning of condensin on mitotic chromosomes

Abstract : The condensin complex is a key driver of chromosome condensation in mitosis. The condensin-dependent assembly of highly compacted chromosomes is essential for the faithful transmission of the genome during cell division. Many independent studies have established that gene transcription impacts the association of condensin with chromosomes, but the molecular mechanisms involved are still unclear. This is especially true as a number of sometimes contradictory mechanisms have been proposed so far. Here, we show in Schizosaccharomyces pombe that condensin accumulates specifically in the vicinity of a subset of RNA polymerase III-transcribed genes in the absence of the conserved DNA/RNA helicase Sen1. We demonstrate that Sen1 is a cofactor of RNA polymerase III (RNAPIII) required for efficient transcription termination. These results are important because they fundamentally challenge the pre-existing view that RNAPIII terminates transcription autonomously. Strikingly, we show that the RNAPIII transcription termination defects are directly responsible for the accumulation of condensin in the absence of Sen1. This indicates that the quality control of transcription impacts the distribution of condensin on mitotic chromosomes. We propose that above a certain density threshold, the accumulation of RNAPIII constitutes a barrier for the translocation of condensin on chromosomes.
Complete list of metadatas

Cited literature [233 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02414560
Contributor : Abes Star :  Contact
Submitted on : Monday, December 16, 2019 - 4:33:07 PM
Last modification on : Monday, January 13, 2020 - 1:11:11 AM
Long-term archiving on: : Tuesday, March 17, 2020 - 7:58:28 PM

File

RIVOSECCHI_Julieta_2019LYSEN04...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02414560, version 1

Citation

Julieta Rivosecchi. Sen1-mediated RNAPIII transcription termination controls the positioning of condensin on mitotic chromosomes. Cellular Biology. Université de Lyon, 2019. English. ⟨NNT : 2019LYSEN041⟩. ⟨tel-02414560⟩

Share

Metrics

Record views

156

Files downloads

188