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Caractérisation fonctionnelle de la protéine ECT2 comme lecteur de la modification N6-méthyladénosine des ARN messagers chez la plante Arabidopsis thaliana

Abstract : Control of gene expression is a crucial process for development, reproduction or acclimation to environmental stresses and involves post-transcriptional regulatory pathways acting on messenger RNAs (mRNAs). These molecules carry chemical modifications of which N6-methyladenosine (m6A) is one of the most abundant. This modification allows notably the recruitment of specific proteins qualified as “readers” which, in mammals, mostly act to promote decay and/or translation of mRNAs. My thesis aimed to characterize the functions of one of these readers, named ECT2, in the model plant Arabidopsis thaliana. First, its binding function to methylated mRNAs and its role in plant development was demonstrated. At the molecular level, a proteomic approach identified numerous ECT2’s protein partners, mainly involved in mRNA metabolism including translation inhibition factors. Results obtained from a translatome analysis suggest a model where ECT2 could play a repressive role on the translation of methylated mRNAs cooperatively with its partners LARP1 and DCP5, two evolutionarily conserved factors acting in translational control of mRNAs. Finally, I also discovered that ECT2 is dynamically modified with phosphorylations in response to heat stress affecting especially its ability to recognize m6A residues. These works suggests for the first time that the activity of an m6A reader could be regulated by phosphorylations in response to environmental changes.
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https://tel.archives-ouvertes.fr/tel-02410927
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Jérémy Scutenaire. Caractérisation fonctionnelle de la protéine ECT2 comme lecteur de la modification N6-méthyladénosine des ARN messagers chez la plante Arabidopsis thaliana. Autre [q-bio.OT]. Université de Perpignan, 2017. Français. ⟨NNT : 2017PERP0033⟩. ⟨tel-02410927⟩

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