Skip to Main content Skip to Navigation
Theses

Etude de la dégénérescence cellulaire épigénétique Crippled Growth chez le champignon Podospora anserina

Abstract : Many fungi display epigenetic instability. In Podospora anserina, the epigenetic cell degeneration “Crippled Growth” (CG) is characterized by slow growth, alteration of pigmentation and inability to differentiate fructifications and aerial hyphae. CG is due to a cytoplasmic and infectious element called C produced during stationary phase. Two types of CG mutants were isolated: IDC mutants impaired in the development of CG and PDC mutants promoting the development of CG. The former have been well studied and all mutants are affected in the MAPK1 pathway, the MAPK2 pathway, the Nox1 NADPH oxidase complex, or in small cysteine-containing proteins probably involved in signal transduction. Here, we present for the first time an analysis of two PDC mutants, the PDC2205 and the PDC2209 strains. PDC2205 is mutated in the PDC1 gene and encodes the PDC1 protein which is conserved in most Ascomycota. This protein regulates CG by inhibiting the PaMpk1 pathway. As for the mutant PDC2209, its analysis has allowed to identify the cluster 2209. This cluster encodes the component of the activation loop of the PaMpk1 and the repressors of the loop. These two types of elements, activators and repressors, seem to be organized in two toxin/antitoxin systems. Besides, this cluster is conserved in the Sordariales and the Chaetosphaeriales with a very specific evolutionary story. So, this cluster is very important, but its function is still unknown.
Document type :
Theses
Complete list of metadatas

Cited literature [209 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02407606
Contributor : Abes Star :  Contact
Submitted on : Thursday, December 12, 2019 - 3:44:07 PM
Last modification on : Saturday, August 29, 2020 - 3:22:38 AM

Identifiers

  • HAL Id : tel-02407606, version 1

Citation

Tinh-Suong Nguyen. Etude de la dégénérescence cellulaire épigénétique Crippled Growth chez le champignon Podospora anserina. Biologie cellulaire. Université Sorbonne Paris Cité, 2018. Français. ⟨NNT : 2018USPCC164⟩. ⟨tel-02407606⟩

Share

Metrics

Record views

124

Files downloads

272