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Particules magnétiques pour le traitement du cancer par effet magnéto-mécanique, application au glioblastome

Abstract : Glioblastoma is a brain cancer with a very poor prognosis. Existing therapies improve only slightly the median survival. In this work, we study a new treatment by magneto-mechanical actuation of particles (TMMAP). A low frequency (20 Hz) rotating magnetic field is applied to stimulate magnetic particles localized near cancer cells. Magnetic particles are produced by a top-down approach in clean room. Permalloy disks with a vortex configuration have a low remanence and are well dispersed in suspension. Multilayers of Co/Pt with a perpendicular anisotropy and permalloy vortex particles with an ellipse shape are also studied. TMMAP efficiency is tested in-vitro on glioblastoma cell line and the parameters are optimized. A huge diminution of living cells and an affected behavior of the remaining cells are observed after treatment. TMMAP is then adapted to an in-vivo study on glioblastoma orthotopic model on nude mice and the intratumoral injection of the particles is developed. Few differences are observed between tissues submitted to TMMAP or injected with particles, and survival is slightly increased. To mimic mechanical properties of the brain in a more relevant model, an in-vitro 3D model is proposed and validated. Based on cells grown as a spheroid and encapsulated in an agarose gel, this model brings optimization tracks.
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Submitted on : Thursday, December 12, 2019 - 8:28:06 AM
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Cécile Naud. Particules magnétiques pour le traitement du cancer par effet magnéto-mécanique, application au glioblastome. Médecine humaine et pathologie. Université Grenoble Alpes, 2019. Français. ⟨NNT : 2019GREAS005⟩. ⟨tel-02406101⟩

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