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, Secondary antibodyes Alexa 488 anti-mouse, Alexa 488 antirabbit, Alexa 594 anti-mouse and Alexa 594 anti-rabbit were purchased from ThermoFisher Scientific, Primary antibodyes rabbit anti-Nestin and rabbit anti-GFAP were purchased from Abcam

. Dapi, P. /. Hepes, N. Strept, . Pyruvate, and . B27, DNAse and ProLong Gold antifade were purchased from Thermo Fisher Scientific, CellTiter 96® AQueous One Solution Cell Proliferation Assay was purchased from Promega

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, Preparation of RA-loaded NFL-LNCs NFL-LNCs. Stok-LNCs were prepared following the protocol developed by

K. Briefly and . Hs15®, Labrafac® (1.028 g) and water (2.962 g) were mixed under gentle magnetic stirring at 30°C for 5 min. The solution was progressively heated (90 °C) and cooled (60 °) three times. During the last cooling, cold water (12.5 g at 4 °C) was added at 72-74 °C under high speed stirring, Lipoïd® (0.075 g), NaCl (0.089 g)

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, Avenue des Constellations 1200

, Citizenship: Italian dario.carradori@uclouvain.be

. Dario-carradori-pharmacist, PhD Student EDUCATION/FORMATION 10/2013-present: Double PhD in Life and Health Sciences at Université d'Angers

, New nanoparticle-based drug delivery systems for neural stem cell targeting and difefrentiation" Thesis director: Dr. Joel Eyer. Thesis co-director: Dr. Anne des Rieux. Supervisors : Prof. Patrick Saulnier and Prof. Véronique Préat Research domain: drug delivery, nanomedicine, neural stem cells, neurodegenerative diseases 09/2006-05/2012: Combined Bachelor and Master in Pharmaceutical Chemistry and Technology at Università degli Studi di Camerino, Nanoparticles for brain targeting" Research domain: drug delivery, nanomedicine, neurodegenerative diseases During this period: Stage (6 months) at Institut Galien Paris-Sud, 2013.

, Design and Realization in animal experimentation, Nantes

, Habilitation to the profession of Pharmacist in Italy

, Alzheimer's disease, blood-brain barrier, nanomedicine 2015 (6 months): Traineeship at Camerino Hospital Pharmacy (Camerino, Italy) RESEACRH FIELDS Drug delivery, Neural stem cells, Neurodegenerative diseases JOB-RELATED SKILLS LANGUAGES: Italian (mother tongue), English (Prof. working proficiency)

. Informatics:-office, G. Imagej, and . Prism, Polymeric synthesis (basic), Fluorescence activated cell sorting, Immunocytochemistry, Immunohistochemistry, Analytical chemistry, qPCR, ELISA, Electrophoresis, Purification methods (dialysis, gel filtration, ultracentrifugation), In vivo experiments (biodistribution, pharmacological efficacy, surgery), Scientific writing

, OTHERS: Networking, Team worker, Fast learner, Independent, Problem-solver, Efficient, Rigorous, Selfcritical

. Scholarships/grants,

, NFL-lipid nanocapsules target neural stem cells from the brain in vitro and in vivo, Mechanistic study on the interaction between PtX and neural stem cell cellular membranes, 2015.

, POSTERS -14 th European Symposium on Controlled Drug Delivery, 2016.

, th Nanofar Autumn School, 2015.