Skip to Main content Skip to Navigation
Theses

Analyse immuno-épidemiologique des anticorps antiparasitaires et rôle du répertoire anticorps auto-réactifs dans la protection contre le Paludisme en Côte d'Ivoire

Abstract : Immune response in malaria is associated either with the protection or as a signature of the infection. Differential immune response, anti-parasitic and self-reactive responses are important phenomena of the host-parasite interaction regarding the pathology and also a source of epidemiological information relevant for monitoring and malaria control. This thesis aims on the one hand, to target potential epidemiological application of measures antibody responses levels to variations in the intensity of transmission in some sites, and also analyze the contribution of self-reactive antibody répertoire in clinical malaria protection in Ivory Coast. Three sentinel sites (Abobo, Korhogo and Man) were geographically diverse recruitment object from symptomatic patients. In Abobo, asymptomatic carriers of parasites children were enrolled to investigate the role of self-reactive répertoire and its relationship with clinical protection from malaria. Recombinant proteins or peptides derived from liver or blood stage antigens of Plasmodium was used to measure antibody response and analyze the relationship between the degree of immunity and epidemiological background in sentinel sites. Human self-reactive antibody répertoires were analyzed by quantitative immunoblotting (panama blot). The analysis of antibody responses using a multiplex immunoassay has shown that the level of immunity may be a differential indicator and a risk criterion with respect to the degree of parasitic infection. Thus measures such as reactivity profiles can contribute positively to differential and scalable integrated monitoring measures against malaria. Furthermore the second part of our study shows the existence of induction of a self-reactive component in asymptomatic carriage of Plasmodium. The Increase of this self-reactive response was associated with a decrease in total IgG response against 3D7 in asymptomatic carriers. Ours results shown that the reactivity of serum IgG against the proteins bands S6, S10, S11, S12, and S13 of brain extract (of molecular weight between 50-75 kDa) discriminate different transmission sites. These bands could harbor host proteins that could be used as epidemiological markers.
Complete list of metadatas

Cited literature [164 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02396382
Contributor : Joseph Djaman <>
Submitted on : Friday, December 6, 2019 - 2:12:19 AM
Last modification on : Friday, October 23, 2020 - 4:57:19 PM
Long-term archiving on: : Saturday, March 7, 2020 - 1:29:04 PM

File

These Koffi David.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-02396382, version 1

Collections

Citation

David Koffi. Analyse immuno-épidemiologique des anticorps antiparasitaires et rôle du répertoire anticorps auto-réactifs dans la protection contre le Paludisme en Côte d'Ivoire. Immunologie. Université Félix Houphouët-Boigny (Abidjan, Côte d'Ivoire), 2017. Français. ⟨tel-02396382⟩

Share

Metrics

Record views

143

Files downloads

691