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STATUT EN VITAMINE D ET POLYMORPHISME DU GENE DE SON RECEPTEUR CHEZ DES PERSONNES VIVANT AVEC LE VIRUS DE L’IMMUNODEFICIENCE HUMAINE (PVVIH) EN CÔTE D’IVOIRE

Abstract : The main goal of this study is to contribute to better care for people living with HIV (PLHIV) in Côte d'Ivoire through the analysis of the allelic polymorphism of the vitamin D3 receptor (VDR) in relationship with 25 (OH) D3 concentrations. To achieve this, an important component of this research is to determine the 25 (OH) D3 and parathyroid hormone status, to describe the polymorphism of 4 portions of the vitamin D3 receptor gene and to analyse the association between the polymorphism of the VDR gene of vitamin D, the concentrations of 25 (OH) D3 and the degree of immunosuppression. This study included 326 blood samples from adult individuals (163 PLHIV and 163 HIV- controls) aged 18 to 49 years of which 50 (32 HIV-positive patients and 18 HIV-negative controls) were used for the study of vitamin D receptor gene polymorphism. The CD4+ lymphocyte count was done by flow cytometry (FacsCalibur). 25 (OH) D3 was determined by high performance liquid chromatography (HPLC). The PCR products obtained after amplification of genomic DNA extracts from peripheral blood mononuclear cells (PBMC) have been purified and sequenced. Mean concentrations of parathyroid hormone were normal in the whole thing. In contrast, a 25 (OH) D3 deficiency was observed in treated patients aged 35 to 49 years with CD4+ less than 200 cells/mm3 under TDF-3TC-EFV or AZT-3TC-NVP and those aged 18 to 25 years under AZT-3TC-NVP. In addition, sufficient levels of 25 (OH) D3 were observed in patients treated with TDF-3TC-LPV/r. The study of the vitamin D3 receptor (VDR) gene has shown that polymorphisms affecting the Fok-1, Bsm-1 and Apa-1 fragments were more common among HIV-positive infected people. The polymorphisms affecting the Fok-1, Bsm-1 and Apa-1 fragments were associated with 25 (OH) D3 concentrations and also with VDR activity. In conclusion, HIV-infected patients on antiretroviral treatment constitute a population at high risk of deficiency or insufficiency of vitamin D3. This disruption of the 25 (OH) D3 concentration could be due to insufficient nutritional intake by foods rich in vitamin D, antiretroviral treatment and polymorphisms affecting the Fok-1, Bsm-1 and Apa-1 fragments and not a defect related to the parathyroid hormone.
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https://tel.archives-ouvertes.fr/tel-02387674
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Submitted on : Saturday, November 30, 2019 - 12:10:07 AM
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Aya Jeanne Armande Ake. STATUT EN VITAMINE D ET POLYMORPHISME DU GENE DE SON RECEPTEUR CHEZ DES PERSONNES VIVANT AVEC LE VIRUS DE L’IMMUNODEFICIENCE HUMAINE (PVVIH) EN CÔTE D’IVOIRE. Biochimie, Biologie Moléculaire. Université Félix Houphouët Boigny (Abidjan, Côte d'Ivoire), 2019. Français. ⟨tel-02387674⟩

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