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EVALUATION DE QUELQUES OLIGOELEMENTS ET DE DEUX ACIDES AMINES ESSENTIELS (Lysine, Thréonine) CHEZ LES PERSONNES VIVANT AVEC LE VIRUS DE L’IMMUNODEFICIENCE HUMAINE (PVVIH) EN CÔTE D’IVOIRE

Abstract : The main goal of this study is to contribute to the care of people living with HIV (PLHIV) in Côte d'Ivoire. An important aspect of this research is to assess trace element profile (copper, zinc and iron) and the essential amino acid status (lysine and threonine) of these people living with HIV/AIDS in Côte d'Ivoire. 254 adults (127 PLHIV and 127 controls) were included in this study. After confirmation of HIV status (DETERMINE® test and GENIE II test), CD4+ T-lymphocytes count was performed using flow cytometry (FacsCalibur). Flame Atomic absorption spectrophotometry (Varian AA20 Pattern®, France) was used to determine serum trace element level. High Performance Liquid Chromatography (HPLC) was used to assess plasma levels of lysine and threonine. Age group (26-49 years) selected in this study was the most exposed to HIV infection in Côte d'Ivoire. HIV-infected people were young (around 37 years old), sexually and economically active. Men and women age average were respectively 40 years and 35 years. This study was also characterized by nutriments systemic homeostasis disruption studied during HIV infection, and it persistence despite appropriate antiretroviral therapy. This disturbance has been reflected by high levels of zinc and copper, with a negative relationship between serum copper levels and CD4 levels. HIV-infected patients with CD4 + count below 200 cells/mm3 were characterized by decreased serum zinc and serum iron high levels. Lysine deficiency and threonine excess were also reported in more than half of the subjects (PLHIV and controls). In HIV-infected men, lysine deficiency was more prevalent, whereas threonine excess was observed in HIV-infected women. However, in HIV-negative controls, threonine excess was common to all men and reduced in half of the women. Correlation between CD4 + T lymphocyte count and lysine was significant and not significant for threonine. In PLHIV, when CD4 + T cell count was below 350 cells/mm3, lysine level was very low and threonine level was normal. In conclusion, high serum zinc level and very marked reduced serum iron level were observed in patients living with HIV compared to seronegative controls. This study revealed a deficiency of lysine and an excess of threonine in more than half of the two types of subjects. Lysine deficiency was more prevalent among male PLHIV, while the excess of threonine was observed in female PLHIV. This lysine deficiency is due to a viral infection and nutritional deficiency of lysine. The level of threonine depends on the degradation of the health status of the subject. Therefore, effective early nutritional management of lysine and threonine is very essential to slow viral replication in order to improve the quality of life of patients living with HIV.
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Laurent Kipré Seri. EVALUATION DE QUELQUES OLIGOELEMENTS ET DE DEUX ACIDES AMINES ESSENTIELS (Lysine, Thréonine) CHEZ LES PERSONNES VIVANT AVEC LE VIRUS DE L’IMMUNODEFICIENCE HUMAINE (PVVIH) EN CÔTE D’IVOIRE. Biochimie, Biologie Moléculaire. Université Félix Houphouët-Boigny, Abidjan (Côte d'Ivoire), 2017. Français. ⟨tel-02358465⟩

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