The average ?Ct value of the control group is subtracted from the sample to derive a ???Ct value. The expression of each gene is evaluated by 2???Ct, according to Livak and Schmittgen, 2001. ,
, List of primers used in this study
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, In order to evaluate the concentration of factors secreted by the cell cultures in every condition, ELISA quantikine kits MG100
, Mouse/Rat IGF-1, DAC00B Human/Mouse Activin A, DGDF80 gdf-8/myostatin, DFN00 from R&D systems are used
, Proteins are denatured with a Bolt kit (Molecular probes, Invitrogen). The running period is 20 min at 200V with the Blot kit, and the transfer period is 20 min at 100V. Membrane of nitrocellulose is incubated with blocking buffer TBS-Tween with 5% not fat milk, Samples are treated with lysis buffer RIPA which contains Tris-Cl 50 Mm pH=7.5, 150Mm NaCl, 1% NP40, 0.5% desoxyclorate de sodium
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