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Rôle du microenvironnement cellulaire de la mégacaryopoïèse

Abstract : The main role of platelets is to stop bleeding. They are produced in the bone marrow by megakaryocytes (MK) that are produced by the differentiation of hematopoietic stem cells (HSC). The objective of my thesis was to identify the cellular elements of the microenvironment controlling megakaryopoiesis. My work has identified a particular population of hepatic progenitors from the fetal liver capable of promoting in vitro the early stages of megakaryopoiesis from human and murine HSC (Brouard et al., 2017). The role of endothelial cells (EC), purified from human bone marrow, in late maturation stages was studied in co-culture experiments with predifferentiated MK. My results show that these EC have the unique property in comparison with EC from other tissues, of promoting the maturation of MK. A differential transcriptional analysis identified possible effectors that could lead to a better understanding of the mechanisms of megakaryopoiesis and improve platelet production in culture.
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Submitted on : Friday, October 25, 2019 - 1:16:08 PM
Last modification on : Friday, August 21, 2020 - 3:39:48 AM
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  • HAL Id : tel-02333479, version 1

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Camille Jost. Rôle du microenvironnement cellulaire de la mégacaryopoïèse. Hématologie. Université de Strasbourg, 2019. Français. ⟨NNT : 2019STRAJ015⟩. ⟨tel-02333479⟩

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