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Cultures multi-parallélisées en millifluidique digitale : diversité et sélection artificielle

Abstract : The role of bacterial communities is essential in ecosystems, but also in the industry, agriculture and human health. Communities’ structure increases their potential applications unlike isolated bacteria. While the culture and engineering of a bacterial strain has become common, currently no technology exists to allow cultivation, handling, selection and evolution of bacterial communities. This work focuses on the design, development and characterization of a tool which can perform evolution of communities using digital millifluidics. With this tool, we handle one thousand cultures of bacteria in drops incubated and analyzed continuously, and the evolution of which we would like to manage. The genetic drift, the genic flows, the diversification and the selection are the four agents of the Evolution which fixes the four axes of this work. The preservation of the diversities, specific to a culture and between them, affect the design of our tool. Its use involves exchanges between cultures which we characterized. We created a protocol allowing to estimate the diversity of these cultures, and thus to discriminate them for robust selection. We finally conceived a system automating their artificial selection: their directed evolution is within easy reach.
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https://tel.archives-ouvertes.fr/tel-02329818
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Submitted on : Wednesday, October 23, 2019 - 4:54:10 PM
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  • HAL Id : tel-02329818, version 1

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Jean-Baptiste Dupin. Cultures multi-parallélisées en millifluidique digitale : diversité et sélection artificielle. Chimie analytique. Sorbonne Université, 2018. Français. ⟨NNT : 2018SORUS127⟩. ⟨tel-02329818⟩

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