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Evaluation de la réponse cellulaire et moléculaire d'une diatomée benthique d'eau douce à l'exposition à des nanoparticules carbonées

Abstract : Different approaches were used to assess the effect of two forms of carbon-based nanoparticles (CNP) nanotubes and graphene, in order to determine the mechanism of the response generated by the benthic freshwater diatom Nitzschia palea. The effect at the cellular community scale demonstrated a temporary impact on biofilm growth and an accumulation of NPC in the extracellular matrix. The use of transcriptomic study evidenced the role of the physic interaction, causing alteration of the frustule, in the extracellular response leading to an overexcretion of exopolymeric substances (EPS). This approach also revealed the impact of NPC on the photosynthetic activity of diatoms and a modification of the energetic metabolism suggesting an energetic allocation for the EPS production. The study of the extracellular proteome allowed to have a first insight of the extracellular matrix composition, in majority composed of hydrophobic-like proteins. In NPC exposure, diatoms seemed to produce an adhesive system allowing to strengthen the extracellular matrix and increase the biofilm stability while trapping NPC. The exposition of diatoms to the two NPC forms induce a response greatly similar for the highest tested concentration.
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Marion Garacci. Evaluation de la réponse cellulaire et moléculaire d'une diatomée benthique d'eau douce à l'exposition à des nanoparticules carbonées. Biologie végétale. Université Paul Sabatier - Toulouse III, 2018. Français. ⟨NNT : 2018TOU30250⟩. ⟨tel-02328953⟩

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