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Contribution à la compréhension des mécanismes moléculaires impliqués dans la virulence de Staphylococcus lugdunensis.

Abstract : Our work has sought to better understand mechanisms involved in Staphylococcus lugdunensis virulence, a species whose pathogenicity is close to that of Staphylococcus aureus. We performed the first functional characterization of a two-component regulatory system, LytSR, in this species. This system has been shown to be involved in the control of major metabolic processes and in virulence. It is indeed involved in biofilm formation, probably in connection with the control of cell death. LytSR is furthermore implicated in the pathogenesis of S. lugdunensis infections, as demonstrated in the infection model of the nematode Caenorhabditis elegans. To better characterize and monitor the diffusion of predominant clones in this species, we have in a second part developed three new typing methods (MLVA, TRST and fbl-typing), based on the polymorphism of variable number of tandem repeats. These methods were highly discriminant, allowing the definition of new genotypes in this clonal species. These tools are very promising for micro- and macro-epidemiological studies in S. lugdunensis, fbl-typing appearing in many ways as the frontline tool (http://fbl-typing.univ-rouen.fr/). Finally, we have shown that the variability of the binding of S. lugdunensis to fibrinogen in vitro can be partly explained by some fbl genetic variations.
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Sandrine Dahyot. Contribution à la compréhension des mécanismes moléculaires impliqués dans la virulence de Staphylococcus lugdunensis.. Sciences agricoles. Normandie Université, 2019. Français. ⟨NNT : 2019NORMR046⟩. ⟨tel-02325654⟩

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