Estimation de position par des techniques d’impédancemétrie : applications aux puces microfluidiques

Abstract : This thesis is part of the general application framework of single cell analysis. In order to improve the selectivity of single cell sorting, FEMTO-ST teams propose to control in closed loop the trajectories of the cells in real time during their journey in the chips dedicated to sorting. In this context, my thesis work focuses on an innovative method for real-time detection of cell position, directly integrated into chips and based on the physical principle of impedance measurement.When a cell passes through a microchannel, it changes the measurable impedance between electrodes placed on the edges of the channel.A generic method for formulating impedance variations as a function of particle position has been proposed (direct model). A method for estimating the position of a particle based on impedance measurements has also been proposed (inverse model). It uses an extended Kalman filter, allowing data from several pairs ofelectrodes to be merged, and exploiting available information such as measurement noise distribution and particle dynamic model. The validation of the method was carried out on an experimental bench that was developed during this thesis and on numerical simulations.This work shows the relevance of using impedance measurement to build a position sensor for particles immersed in a microchannel. This method is an alternative to the use of optical microscopes and has the advantage of being very compact.
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Benoit Brazey. Estimation de position par des techniques d’impédancemétrie : applications aux puces microfluidiques. Mécanique des fluides [physics.class-ph]. Université Bourgogne Franche-Comté, 2019. Français. ⟨NNT : 2019UBFCD011⟩. ⟨tel-02313938⟩

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