Rôle des acides aminés dans la limitation de l’adiposité sous régime hyperprotéique.

Abstract : Several studies have reported that some kinases located in the liver respond to the availability of amino acids. These kinases are mammalian target of rapamycin '(mTOR), "adenosine monophosphate-activated protein kinase" (AMPK) and "general control non-depressible kinase 2" (GCN2).The aim of our study was to clarify the role of two of these signaling pathways, AMPK and GCN2 in the adaptations of energy and protein metabolism in response to the modulation of dietary protein content. Wild-type and liver AMPK-deficient or liver GCN2-deficient mice were fed either a low, a normal or high protein diet during three weeks. Analyzes showed that liver AMPK-deficient mice fed under a normo-protein diet exhibit an adapatation of liver metabolism and secret FGF21 which enables them to have normal postprandial oxidation profiles.In contrast, liver AMPK-deficient mice fed a low or a high protein diet exhibit an alteration in postprandial oxidation profiles. The deletion of GCN2 in the liver only has an effect under low protein diet as liver GCN2 deficient mice have a lower lipid oxidation and a higher carbohydrate oxidation linked to the absence of FGF21 secretion. Concerning protein metabolism, AMPK and GCN2 do not seem to be involved in protein synthesis rate in the posrprandial period in the liver and periphery in the postprandial muscle. In conclusion, these studies show that hepatic AMPK and GCN2 deletions affect energy metabolism, but not protein metabolism and that the consequences depend on diet composition.
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Tristan Chalvon-Demersay. Rôle des acides aminés dans la limitation de l’adiposité sous régime hyperprotéique.. Médecine humaine et pathologie. Université Paris-Saclay, 2016. Français. ⟨NNT : 2016SACLA018⟩. ⟨tel-02303251⟩

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