Développement de la spectrométrie de masse MALDI -TOF pour l'identification des champignons filamenteux d'intérêt alimentaire et étude de leur résistance aux molécules biocides

Abstract : Spoilage fungi represent a major cause of food and economic losses and certain species, which may produce mycotoxins, may also pose a threat to human and animal health. Thus, food safety and quality management relies notably on a good knowledge of the involved species. This knowledge is notably based on their fast and reliable identification and on the study of abiotic factors affecting their growth such as food preservatives, which are commonly used in the food industry. In this context, the objectives of this PhD. thesis were to develop MALDI-TOF mass spectrometry for mold identification and to evaluate its potential for species complex differentiation and strain typing, and finally, to evaluate the use of laser nephelometry to monitor fungal growth in the presence of food preservatives.First, a robust database was developed with 6500 spectra corresponding to 136 spoilage fungi. Then, MALDI-TOF MS was successfully applied to differentiate 23 species of Aspergillus section Flavi and Penicillium roqueforti isolates belonging to 3 genetically distinct populations.Finally, in 14 fungal species, laser nephelometry allowed a high-throughput monitoring of their growth after exposition to 3 different food preservatives and the determination of their associated minimal inhibitory concentrations.Overall, the obtained results demonstrate the usefulness of alternative techniques for identification and characterization of food spoilage fungi.
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Laura Quero. Développement de la spectrométrie de masse MALDI -TOF pour l'identification des champignons filamenteux d'intérêt alimentaire et étude de leur résistance aux molécules biocides. Microbiologie et Parasitologie. Université de Bretagne occidentale - Brest, 2018. Français. ⟨NNT : 2018BRES0109⟩. ⟨tel-02303042⟩

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