Elaboration et études des propriétés de films minces de carbone amorphe azoté (a-CNx) sur verre conducteur : vers l'élaboration de support en verre polarisable pour la détection électrochimique et/ou optique en puce microfluidique.

Abstract : Electrochemical combined with optical detection in microchip requires the mutual use of transparent and conductive materials onto which the process of oxidation/ reduction of the solvent are minimized over a wide range of potential and must also have a good electrochemical reactivity with respect to species to be detected. That is for these reasons that transparent and conductive glass slides (eg glass/ITO) are among the best candidates. However, the potential window available on these slides remains limited. We have developed protocols for deposition of amorphous carbon nitride (a-CNx) with different nitrogen content sputtered on conductive glass slides and complies fully with the above two requirements? These polarizable glass slides were characterized in air and in solution. The wide range of conductivity obtained with these materials destined them for different applications in microfluidics : to modulate the electroosmotic flow in electrophoresis or as microelectrode detection microchips. The results also show that it is possible to electrochemically activate or regenerate the reactivity of th glass/ITO/a-CNx electrodes and thereby avoid polishing. The efficiency of the activation of the glass/ITO/a-CNx electrodes was demonstrated for the electrochemical characterization of a fluorescent label, 2,3-naphatlène dicarboxyaldehyde (NDA), and the detection of different amino acids and peptide derivatives of the transthyretin by the NDA. Finally, preliminary results with a specific two microelectrodes configuration show that it is possible to derive and detect Tranthyretin in microchip
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Mathilde Faure. Elaboration et études des propriétés de films minces de carbone amorphe azoté (a-CNx) sur verre conducteur : vers l'élaboration de support en verre polarisable pour la détection électrochimique et/ou optique en puce microfluidique.. Matériaux. Université Pierre et Marie Curie (Paris VI), 2013. Français. ⟨NNT : 2013PA066820⟩. ⟨tel-02302169⟩

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