Identification de voies neuroendocriniennes du contrôle de la physiologie chez l'huître Crassostrea gigas par la caractérisation fonctionnelle de couples ligands/récepteurs

Abstract : The neuroendocrine regulators of the physiology of Lophotrochozoa, the sister clade of Ecdysozoa among Protostoma, remain poorly understood. Thanks to the recent emergence of genomic, transcriptomic and peptidomic resources in the Pacific oyster Crassostrea gigas, the functional characterization of ligand/receptor pairs regulating a diversity of physiological functions has been facilitated. Using a reverse endocrinology approach, a number of orphan G Protein-Coupled Receptors (GPCRs) have been functionally characterized. Three signalling systems have been studied in the oyster: The cholecystokinin/sulfakinin (CCK/SK), the calcitonin (CT) and the dopamine (DA) signalling systems. Two CCK/SK receptors and ligands have been characterized. In vitro bioassays and feeding conditions suggested the potential involvement of this signalling system in the regulation of digestion and satiety. Besides, two couples of CT-type peptides and receptors have been characterized showing, as for their vertebrate counterparts, their possible role in the regulation of water and ion balance. A receptor specifically activated by dopamine and by tyramine has also been characterized. This signalling system appeared to be implicated in the mediation of stress and to play a role in the regulatory processes of reproduction in the gonads. This study allowed the characterization in the oyster of ligand receptor pairs homolog to known signalling systems present in Ecdysozoa and vertebrates, thus confirming the origin of these neuroendocrine systems in the common ancestor of Bilateria. The results of this study also contributed to understand how the oyster integrates external parameters and adapts to various environmental constrains.
Document type :
Theses
Complete list of metadatas

Cited literature [547 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02299481
Contributor : Abes Star <>
Submitted on : Friday, September 27, 2019 - 5:34:09 PM
Last modification on : Saturday, September 28, 2019 - 1:24:39 AM

File

sygal_fusion_28103-schwartz-ju...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02299481, version 1

Citation

Julie Schwartz. Identification de voies neuroendocriniennes du contrôle de la physiologie chez l'huître Crassostrea gigas par la caractérisation fonctionnelle de couples ligands/récepteurs. Biologie animale. Normandie Université, 2019. Français. ⟨NNT : 2019NORMC203⟩. ⟨tel-02299481⟩

Share

Metrics

Record views

247

Files downloads

20