Diversité et bases moléculaires de l’agressivité de Ganoderma Boninense, agent causal de la pourriture basale du stipe chez le palmier à huile (Elaeis guineensis)

Abstract : The understanding of genetic structuration and population dynamic, as well as the molecular mechanisms ruling host/pathogen interaction, are key elements for disease management. During this study, as first step, we were looking to develop microsatellites markers from genomic data obtained from sequencing of a Ganoderma boninense pure strain. Those markers allowed studying genetic structuration and demographic history of G. boninense. Genotyping of a subset of samples from sampling mission in Malaysia and Sumatra have highlighted two main groups in South-East Asia. Then, we focused on a region gathering peninsular Malaysia and Sumatra together, as it is both historical region of industrial oil palm culture development and the first region of basal stem rot observation caused by G. boninense, and to appear as a single population. We examine this region at a lower scale in order to highlight a potential genetic substructure in this population. We tested for effect of genetic tree background of each sample, number of planting generation before sampling and geographical distance between sample in order to observe a potential correlation between genetic substructure and one of those factors. As no correlation appeared, we concluded that this population does not have a genetic substructure. On the other hand, the comparison between several demographic evolution scenarios have shown a strong support for a past expansion event further back in time from the beginning of industrial oil palm culture development. To conclude, the exploration of transcriptomic data between strains owning aggressive or non-aggressive profile showed the differential expression of a hundred genes owning a functional annotation. Results from both approaches will allow the development of better disease management and a better resistance selection and management program.
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Maxime Mercière. Diversité et bases moléculaires de l’agressivité de Ganoderma Boninense, agent causal de la pourriture basale du stipe chez le palmier à huile (Elaeis guineensis). Génétique des plantes. Université Montpellier, 2015. Français. ⟨NNT : 2015MONTS263⟩. ⟨tel-02299106⟩

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