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In-vivo studies of malformations of cortical development associated with mutations in TUBG1

Abstract : Missense heterozygous variants in the gamma tubulin gene TUBG1 have been linked to malformations of cortical development, associated with intellectual disability and epilepsy. Here, we investigated through in-utero electroporation and in-vivo studies, how four of these variants affect cortical development. We show that TUBG1 mutants affect neuronal positioning within the cortical wall, by a disrupting the locomotion of newly born neurons but without affecting neurogenesis. We propose that mutant γ-tubulin affects overall functioning of γ-tubulin complexes, and in particular their role in the regulation of microtubule dynamics. Additionally, we developed a knock-in Tubg1Y92C/+ model and assessed consequences of the mutation on cortical development, neuroanatomical features and behaviour. Mutant mice present with global microcephaly, neocortical and hippocampal abnormalities, behavioural alterations and epileptic susceptibility. Thus, we show that Tubg1Y92C/+ mice partially mimic the human phenotype and therefore represent a relevant model for further investigations of the physiopathology of malformations of cortical development.
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Submitted on : Monday, September 23, 2019 - 3:58:49 PM
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Ekaterina Ivanova. In-vivo studies of malformations of cortical development associated with mutations in TUBG1. Neuroscience. Université de Strasbourg, 2018. English. ⟨NNT : 2018STRAJ091⟩. ⟨tel-02294645⟩



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