Séquençage par couplage de spectrométrie de masse et spectroscopie infrarouge de fragments de glycosaminoglycanes

Abstract : Carbohydrates are among the three major classes of biopolymers found in nature. They represent 75% of the biomass. Glycosaminoglycans are carbohydrates, they are present on the surface of cells and are responsible for cell signaling. They are important in many biological processes. Given their importance in biology and health, it is necessary to understand their mechanism. In this thesis we studied two types of glycosaminoglycan tetrasaccharides (chondroitin sulfate and dermatan sulfate) with a new method that combines mass spectrometry with vibrational spectroscopy (MS / IR). We have shown that with this method the signature of the OH-fingerprint works for glycosaminoglycans which have COO- groups and sulfate groups in contrast to simple carbohydrates. This method also validated the relevance of sequencing for the elucidation of sulfate profiles and the nature of hexuronic in oligosaccharides of glycosaminoglycans. The sequencing approach significantly improves the structural resolution compared to the simple spectroscopic analysis of the precursor ion. It provides more information on oligosaccharides. And finally we proposed a protocol for the analysis of mixtures to determine the ratio of the different elements present in the mixture
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Gina Renois Predelus. Séquençage par couplage de spectrométrie de masse et spectroscopie infrarouge de fragments de glycosaminoglycanes. Chimie analytique. Université de Lyon, 2019. Français. ⟨NNT : 2019LYSE1185⟩. ⟨tel-02294549⟩

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