Panton–Valentine leucocidin colocalized with retinal neurons cells and incited early retinal inflammation through rabbit endophthalmitis and retinal explant models

Abstract : Staphylococcus aureus is responsible for many infections. It secretes various virulence factors aggravating the staphylococcal infections. Panton-Valentine leucocidin (PVL) is a virulent leukotoxin from S. aureus and presents active effects towards leukocytes and neuronal cells via the C5a receptor (C5aR). The effects of PVL on retina is little known. We explored PVL retinal cell target and early retinal inflammation and tried to find the processes of bacterial toxins aggravating bacterial endophthalmitis. We employed two different rabbit models to study the PVL effects on retina: intravitreal injection in vivo and retinal explant ex vivo. In the two models, retinal ganglion cells were the only retinal neurons which express C5aR and the major cell targets of PVL in retina. PVL induced retinal Müller and microglial cell activation. The retinal explants were easily manipulated and showed obvious cellular targets of PVL and glial cell activations, they can contribute to research the effects of PVL on retina in future. PVL alone without S. aureus could induce great retinal inflammation after targeting specifically retinal neurons.
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Xuanli Liu. Panton–Valentine leucocidin colocalized with retinal neurons cells and incited early retinal inflammation through rabbit endophthalmitis and retinal explant models. Bacteriology. Université de Strasbourg, 2018. English. ⟨NNT : 2018STRAJ076⟩. ⟨tel-02285920⟩

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