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, Documents relatifs à la thèse 3.1. Mode d'emploi et recommandations pour l

, Toute prise de nourriture, de boisson, de chewing-gum ou l'utilisation de dentifrice doit être évitée dans les 30 minutes précédant le prélèvement. Sinon, il est recommandé de se rincer la bouche avec

. Au-delà-de-cette-durée,

, Il est recommandé de mettre le salitube dès le recueil soit au réfrigérateur

, Vortexer les tubes contenant les standards et les contrôles

, Centrifuger les tubes contenant les échantillons à doser (ici, la salive)

, Déposer 100 µL de chaque standard et 100 µL des 2 contrôles (low et high), Dupliquer tous les dépôts

, Déposer 100 µL de chaque échantillon. Dupliquer tous les dépôts

, Déposer 200 µL d'enzyme conjuguée dans tous les puits (standard, contrôles et échantillons)

, Vider la plaque (retourner d'un coup sec)

, Répéter le 7) 2 fois. La dernière fois, bien essorer la plaque sur du papier absorbant pour enlever tout le liquide)

, Déposer 200 µL de solution substrat dans chaque puits

, Stopper la réaction en déposant 100 µL de solution stop dans chaque puits

, Déterminer l'absorbance à 450 nm à l'aide d'un spectrophotomètre

, Le cortisol entre en compétition avec l'enzyme conjuguée donc moins l'échantillon est coloré et plus il y a de cortisol