Nouveaux hydrogels à base de polysaccharide obtenus par voie biomimétique ou par photoréticulation.

Abstract : In the framework of an eco-responsible context and to take advantage of biocompatibility, notably in cosmetic and biomedical applications, we have developed new hydrogels based on neutral and anionic polysaccharides using two original routes. The first approach is biomimetic and consists of mimicking a natural development of hydrogels that is found in certain plants for which an enzyme, laccase, allows to create crosslinks by dimerization of phenolic compounds, in occurrence of ferulic acid (FA) present on arabinoxylans mucilage of cereal seeds for example. Thus, our work consisted in grafting ferulic acid via two different chemical ways that means imidazole and carbodiimide respectively for neutral or anionic polysaccharides. We functionalized three polysaccharides: pullulan or PUL (neutral model), carboxymethylpullulane or CMP (model anionic) and hyaluronic acid or HA (anionic of interest) with grafting rates of between 2 and 25%. The physicochemical study in diluted and semi-diluted regimes evidenced an associative behavior due to the amphiphilic character of the functionalized polysaccharides. The crosslinking in the presence of laccase, followed in situ thanks to rheology, has been successfully performed on the various envisaged systems with possible controls of kinetics, the final mechanical properties or the swelling of the hydrogels as a function of the neutral or charged nature of the polysaccharides, the degree of substitution in FA, the polymer concentration or the enzymatic activity. The synthesized derivatives have generally demonstrated interesting biological activities (antioxidant and cytocompatibility). The second approach is based on the possible photocrosslinking of polysaccharides (PUL, CMP and HA) functionalized by the grafting of mono or polyunsaturated fatty amine/acid (oleylamine, oleic acid and linoleic acid) via imidazole chemistry. If pullulan grafted with 2% of linoleic acid was found to be water-insoluble due to its neutral character, all other derivatives (i.e. anionic ones) with grafting rates of 3 and 10% showed good solubility in water. The physicochemical studies show a very strong associative character of these amphiphilic derivatives with the formation of physical gels in semi-diluted regime. Photocrosslinking has been demonstrated in situ thanks to rheology/UV irradiation in the presence of a Darocur 1173® photoinitiator. The preliminary results according to this photocrosslinking approach thus open interesting perspectives.
Document type :
Theses
Complete list of metadatas

Cited literature [161 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02272694
Contributor : Abes Star <>
Submitted on : Wednesday, August 28, 2019 - 9:16:08 AM
Last modification on : Thursday, September 5, 2019 - 10:12:06 AM

File

ahdihadrich.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02272694, version 1

Citation

Ahdi Hadrich. Nouveaux hydrogels à base de polysaccharide obtenus par voie biomimétique ou par photoréticulation.. Polymères. Normandie Université, 2019. Français. ⟨NNT : 2019NORMR017⟩. ⟨tel-02272694⟩

Share

Metrics

Record views

92

Files downloads

45