Nouvelle stratégie d'amélioration de la productivité végétale en condition de stress environnemental via un meilleur contrôle du cycle cellulaire

Abstract : Salt stress is one of the main environmental factors limiting plant growth and yield in cereal crops. It is therefore imperative to develop varieties more tolerant to salt stress in order to increase yield and ensure food security. The signaling pathway linking salt stress perception to cellular response was addressed here by studying RSS1-like proteins in plants. RSS1 (Rice Salt Sensitive 1) protein plays an important role in salt stress tolerance. It acts at the interface of stress perception and developmental control and division in meristems. During this work, the RSS1 counterpart named TdRL1 (Triticum durum RSS-Like 1) was isolated from the durum wheat Tunisian variety "Oum Rabiaa". We have demonstrated that TdRL1 carries the conserved D and DEN-Box motifs involved in the post-translational regulation of the protein. In addition, we show that TdRL1 is the functional homologue of RSS1 since it was able to complement the loss-of-function mutant rss1, hypersensitive to salt stress. In addition, heterologous expression of TdRL1 enhances salt stress tolerance in yeast and in Arabidopsis by increasing germination and reducing the accumulation of reactive oxygen species. Our cytological studies have shown that the TdRL1 protein is cytoplasmic in interphase and is localized at the spindle during mitosis. Remarkably, TdRL1 changes its subcellular localization under salt stress treatment and shows a partial accumulation in the nucleus, highlighting the multifunctional nature of this protein during salt stress response. Our data suggest that under salt stress, TdRL1 plays a role in the regulation of the cell cycle in relation with the microtubule network. Pursuing the study of RSS1-like multifunctional proteins will open up new research areas for the creation of wheat varieties that are more resilient to environmental stresses.
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Habib Mahjoubi. Nouvelle stratégie d'amélioration de la productivité végétale en condition de stress environnemental via un meilleur contrôle du cycle cellulaire. Génétique des plantes. Université de Strasbourg; Université de Sfax (Tunisie), 2018. Français. ⟨NNT : 2018STRAJ059⟩. ⟨tel-02272352⟩

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