Regulation of transcription : structural studies of an RNA polymerase elongation complex bound to transcription factor NusA

Abstract : Transcriptional pausing by RNA polymerases (RNAPs) is a key mechanism to regulate gene expression in all kingdoms of life and is a prerequisite for transcription termination. The essential bacterial transcription factor NusA stimulates both pausing and termination of transcription, thus playing a central role. Here, I present single-particle electron cryo-microscopy (cryo-EM) reconstructions of NusA bound to paused elongation complexes with and without a pause-enhancing hairpin in the RNA exit channel. The structures reveal four interactions between NusA and RNAP that suggest how NusA stimulates RNA folding, pausing, and termination. An asymmetric translocation intermediate of RNA and DNA converts the active site of the enzyme into an inactive state, providing a structural explanation for the inhibition of catalysis. Comparing RNAP at different stages of pausing provides insights on the dynamic nature of the process and the role of NusA as a regulatory factor.
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Xieyang Guo. Regulation of transcription : structural studies of an RNA polymerase elongation complex bound to transcription factor NusA. Genomics [q-bio.GN]. Université de Strasbourg, 2018. English. ⟨NNT : 2018STRAJ071⟩. ⟨tel-02269303⟩

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