Lanthanide Energy Transfer Donors on Nanoparticles Surfaces : From Fundamental Mechanisms to Multiplexed Biosensing

Abstract : Optical multiplexing based on nanoparticles provides many advantages for multiparameter biosensing and imaging. However, the changes in one parameter also lead to changing of other parameters, and thus, color, lifetime, or intensity could not be used as an independent parameter, respectively. This thesis can be divided into two aspects. The first one focuses on developing time-resolved single-nanoparticle multiplexing based on Förster resonance energy transfer (FRET) from lanthanide complexes to quantum dot (QD) to fluorescent dyes. Systematical investigation of all different combinations with a broad range of numbers of donors and acceptors on QD are presented, and the experimental results are compared with theoretical modelling. The result do not only contribute to a full understanding of such complicated multi donor-acceptor energy transfer pathways on nanoparticles but also open the opportunity to use the models for developing new strategies to achieve the QD with independent tunable color, lifetime and intensity. The second aspect focuses on the energy transfer mechanism from Tb to gold nanoparticle (AuNP). Nanosurface energy transfer (NSET) proved to be an operational mechanism in PL quenching by AuNPs, which is important information for the development, characterization, and application of nanobiosensors based on PL quenching by AuNPs.
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Chi Chen. Lanthanide Energy Transfer Donors on Nanoparticles Surfaces : From Fundamental Mechanisms to Multiplexed Biosensing. Micro and nanotechnologies/Microelectronics. Université Paris-Saclay, 2019. English. ⟨NNT : 2019SACLS196⟩. ⟨tel-02269108⟩

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