Characterization of the nuclear RNase P of Candida glabrata and improvement of genome editing tools

Abstract : Candida glabrata is an opportunistic pathogenic yeast, and is today the second causative agent of candidemia in Europe and North America. This yeast has many genomic peculiarities such as the presence of new structural domains within ubiquitous non-coding RNAs. The first aspect of this thesis was the study of the atypical RNA subunit of the nuclear Ribonuclease P of C. glabrata. This RNA contains three large additional domains giving the transcript an overall size more than three times larger than the average eukaryotic RNase P RNA subunits. The experiments performed led to a better understanding of the role of these additional domains and demonstrated for the first time the presence of the Rcl1 protein within the RNase P complex. Secondly, this thesis work also contributed to the improvement of existing genome editing tools in C. glabrata. New small and positively selectable integrative cassettes have been developed. These elements exhibited all the required characteristics for their use in wild-type strains and clinical isolates of C. glabrata.
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Yacine Dahman. Characterization of the nuclear RNase P of Candida glabrata and improvement of genome editing tools. Genomics [q-bio.GN]. Université de Strasbourg, 2018. English. ⟨NNT : 2018STRAJ053⟩. ⟨tel-02268678⟩

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