Interactions between coronary artery endothelial cells and leukocyte MPs shed in response to E. coli lipopolysaccharide : in-vitro and ex-vivo studies of the impact of vascular ageing and of high glucose

Abstract : Microparticles (MP) are plasma membrane vesicles shed from stimulated cells. We investigated whether leukocyte MP extracted from rat spleen are reliable markers of aging and effectors of high glucose (HG)-induced endothelial senescence and dysfunction. Data indicate that ageing enhances MP shedding from spleen cells of middle-age and aged rats and raises MP release in response to LPS, or to PMA and ionophore A23187. Of note, MP from aged but not young rats induced senescence of porcine coronary artery primary endothelial cells. In young rats, MPLPS, MPPMA/I but not from resting cells (MPCTL) reduced the endothelial-dependent relaxation of coronary artery rings (CAR) in response to bradykinin with down-regulation of eNOS, up-regulation of COX-2, ICAM-1, VCAM-1. HG enhanced early and late MP release from spleen cells. Prolonged exposure to HG potentiated endothelial dysfunction in CAR and altered vasoconstriction in response to U46619l in a SGLT1/2 and EDHF dependent manner.
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Raed Adill Hannon Altamimy. Interactions between coronary artery endothelial cells and leukocyte MPs shed in response to E. coli lipopolysaccharide : in-vitro and ex-vivo studies of the impact of vascular ageing and of high glucose. Immunology. Université de Strasbourg, 2018. English. ⟨NNT : 2018STRAJ024⟩. ⟨tel-02268618⟩

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