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Modélisation in vitro de la colonisation à staphylococcus aureus ; interactions avec l’infection à rhinovirus

Abstract : Some respiratory viruses such as rhinoviruses seem to promote staphylococcus aureus colonization. However, the details of the bacterial and cellular mechanisms involved in this synergy have not been sufficiently elucidated. The aim of this thesis was to develop and validate an in vitro model mimicking s. aureus colonization of the nasal vestibule by using hacat human keratinocytes. This model allowed to study (i) the adhesion and internalization capacities of various clinical s. aureus strains, (ii) the intracellular efficiency of the antimicrobial molecules used for s. aureus nasal decolonization, (iii) the effect of clarithromycin on rhinovirus infection, and (iv) the impact of rhinovirus infection and non-specific inflammation on s. aureus colonization. This work has mainly identified a new alternative mechanism for the internalization of s. aureus through the binding between the bacterial protein eap (extracellular adherence protein) and the cell receptor icam-1 (intracellular adhesion molecule 1). This alternative pathway is favored in case of rhinovirus infection or inflammation; which could explain the clinical observations of the increase of the load of s. aureus or the risk of infection by this bacterium during respiratory viral infections or post-traumatic inflammations. The results of this thesis illustrate the complexity of the interactions between the mucosal epithelial cells, s. aureus and viral pathogens and suggest that other studies are needed to propose appropriate preventive or therapeutic strategies.
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Submitted on : Tuesday, July 9, 2019 - 5:22:24 PM
Last modification on : Thursday, November 21, 2019 - 2:17:32 AM


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  • HAL Id : tel-02178370, version 1



Mohamed Fedy Morgene. Modélisation in vitro de la colonisation à staphylococcus aureus ; interactions avec l’infection à rhinovirus. Médecine humaine et pathologie. Université de Lyon, 2018. Français. ⟨NNT : 2018LYSES054⟩. ⟨tel-02178370⟩



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