Interactions entre résistance induite chez Solanum tuberosum et traits d’histoire de vie et effecteurs de Phytophthora infestans

Abstract : The management of Phytophthora infestans, responsible for potato late blight, requires the application of about 15 fungicide treatments per cropping season. To reduce the use of pesticides, combining induced resistance and quantitative resistance could be a positive strategy. However, this method requires a better understanding of the interactions between Solanum tuberosum physiological responses and P. infestans ecology. To this end, defense responses induced in potato have been opposed to the pathogen life history traits and effectors. Four potato genotypes with different resistance levels were treated with a concentrated culture filtrate (CCF) of P. infestans inducing PAMPtriggered immunity (PTI). Then, detached leaflets were inoculated with fast- or slow-growing strains of P. infestans.The expression of 14 defense genes and the expression of 6 effectors were analyzed simultaneously by qRT-PCR. Disease symptoms were measured either conventionally or by visible and fluorescence image analysis. The results obtained show that the reduction of symptoms after induction of PTI was specific to the genotype-strain pair. Indeed, the effectiveness of induced defenses by CCF depends on either the escape (growth rate) or adaptation (effectors) strategies of P. infestans and on the genotype inductibility potential (expression of PR proteins). Thus, to optimize the use of induced resistance, it would be necessary to breed inducible genotypes that are able to modulate strains growth rate.
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Cécile Thomas. Interactions entre résistance induite chez Solanum tuberosum et traits d’histoire de vie et effecteurs de Phytophthora infestans. Biologie végétale. Agrocampus Ouest, 2019. Français. ⟨NNT : 2019NSARC141⟩. ⟨tel-02172159⟩

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