Onco-fertilité : Impact des facteurs influençant la réserve ovarienne après chimiothérapie

Abstract : Chemotherapy induces infertility by exerting a direct toxicity on the ovaries, resulting in a depletion of the follicular stockpile. Cyclophosphamide (Cy), widely used for breast cancer, is recognized as one of the most gonadotoxic agent. Recently, it has been proposed that Cy gives rise to follicular depletion by a massive growth of resting follicles which are then destroyed. Since Anti-Müllerian Hormone (AMH) is one of the factors regulating primordial follicles activation, we hypothesized that this hormone might limited Cy-induced gonadotoxicity. We have shown in pubertal mice that recombinant AMH injections are able to preserve primordial follicle loss Cy-induced and might improve fertility outcome after treatment. In addition, we provide evidence that autophagy could be one of the mechanisms involved in the inhibition of follicular recruitment by AMH. At least, nowadays, the “gold standard” method of evaluating ovarian reserve in mouse is a process particularly time-consuming and tedious. We developed a new methodology of automatic primordial follicles detection and counting within mouse ovaries, using modern artificial intelligence methods, especially deep learning approach.
Document type :
Theses
Complete list of metadatas

Cited literature [554 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02170510
Contributor : Abes Star <>
Submitted on : Tuesday, July 2, 2019 - 1:20:59 AM
Last modification on : Tuesday, July 16, 2019 - 2:46:06 PM

File

74349_SONIGO_2018_diffusion.pd...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02170510, version 2

Collections

Citation

Charlotte Sonigo. Onco-fertilité : Impact des facteurs influençant la réserve ovarienne après chimiothérapie. Biologie de la reproduction. Université Paris-Saclay, 2018. Français. ⟨NNT : 2018SACLS184⟩. ⟨tel-02170510v2⟩

Share

Metrics

Record views

238

Files downloads

17