Process development for symbiotic culture of Saccharomyces cerevisiae and Chlorella vulgaris for in situ CO2 mitigation

Abstract : Yeast and microalgae are microorganisms widely studied for the production of high-value compounds used in food and energy area. This work proposes a process of mixed culture of Saccharomyces cerevisiae and Chlorella vulgaris for both growth and CO2 mitigation. The process relies on mutual symbiosis between the two organisms through gas exchange, which is possible by engineering the co-dominance of populations. The two populations must be balanced in such a way so that microalgae can cope with the rate of CO2 production by the yeast activity. The process is performed in non-aerated 5l-photo-bioreactor fitted with a fermentation lock to prevent gas exchange with the outside atmosphere. With this set-up, the CO2 is produced in dissolved form and is available to the microalgae avoiding degassing and dissolution phenomena. The two organism populations are balanced at approximately 20 millions cells per ml, 12% CO2 produced by yeast was reutilized by microalgae within 168 hours of culture. A yeast and microalgae growth model in mixed culture is developed by combining each individual growth model. The predictive yeast model considers the possible metabolic pathways involved in fermentation and respiration and imposes limitation factors on these pathways, in this manner, the model can predict the partition of these pathways. The microalgae individual model is based on the photosynthetic activity. The results of this work show the feasibility of such process and could provide a basis for the development of a green process of low environmental impact.
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Angéla La. Process development for symbiotic culture of Saccharomyces cerevisiae and Chlorella vulgaris for in situ CO2 mitigation. Chemical and Process Engineering. Université Paris-Saclay, 2019. English. ⟨NNT : 2019SACLC031⟩. ⟨tel-02168279⟩

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