S. B. Adler, Reference Electrode Placement in Thin Solid Electrolytes, Journal of the Electrochemical Society, vol.149, 2002.

. Akselrod, Laser-guided assembly of heterotypic three-dimensional living cell microarrays, Biophysical Journal, vol.91, pp.3465-3473, 2006.

. Albrecht, Geometric and material determinants of patterning efficiency by dielectrophoresis, 2004.

, Biophysical Journal, vol.87, pp.2131-2147, 2004.

. Albrecht, Probing the role of multicellular organization in threedimensional microenvironments, Nature Methods, vol.3, pp.369-375, 2006.

T. Allen, Powder sampling and particle size measurement, Particle Size Measurement, vol.1, 1997.

. Altschuler, S. J. Wu-;-altschuler, and L. F. Wu, Cellular heterogeneity: do differences make a difference?, Cell, vol.141, pp.559-563, 2010.

. Arnold, Surface Conductance and Other Properties of Latex-Particles Measured by Electrorotation, Journal of Physical Chemistry, vol.91, pp.5093-5098, 1987.

W. M. Arnold and U. Zimmermann, ElectroRotation -Development of a Technique for Dielectric Measurements on Individual Cells and Particles, Journal of Electrostatics, vol.21, pp.151-191, 1988.

K. Asami, Characterization of heterogeneous systems by dielectric spectroscopy, Progress in Polymer Science, vol.27, pp.15-16, 2002.

. Asami, Dielectric behavior of budding yeast in cell separation, Biochimica Et Biophysica Acta-General Subjects, vol.1381, pp.234-240, 1998.

. Asami, Real-time monitoring of yeast cell division by dielectric spectroscopy, Biophysical Journal, vol.76, pp.3345-3348, 1999.

. Asami, Progression of cell cycle monitored by dielectric spectroscopy and flow-cytometric analysis of DNA content, Yeast, vol.16, pp.1359-1363, 2000.

. Ashkin, Observation of a single-beam gradient force optical trap for dielectric particles, Optics Letters, vol.11, 1986.

. Ashkin, Optical Trapping and Manipulation of Single Cells Using Infrared-Laser Beams, Nature, vol.330, pp.769-771, 1987.

. Ayliffe, Electric impedance spectroscopy using microchannels with integrated metal electrodes, Journal of Microelectromechanical Systems, vol.8, pp.50-57, 1999.

. Badshah, Development of a rapid cure polydimethylsiloxane replication process with near-zero shrinkage, Journal of Micro-Nanolithography Mems and Moems, vol.13, 2014.

. Benazzi, Discrimination and analysis of phytoplankton using a microfluidic cytometer, Iet Nanobiotechnology, vol.1, pp.94-101, 2007.

P. D. Benya and J. D. Shaffer, Dedifferentiated Chondrocytes Reexpress the Differentiated Collagen Phenotype When Cultured in Agarose Gels, Cell, vol.30, pp.215-224, 1982.

H. C. Berg, Random walks in biology, 1993.

. Bhagat, Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies, TRANSDUCERS 2007 -2007 International Solid-State Sensors, Actuators and Microsystems Conference, vol.8, pp.280-286, 2007.

. Brazey, Impedance-based real-time position sensor for lab-on-a-chip devices, Lab on a Chip, vol.18, pp.818-831, 2018.
URL : https://hal.archives-ouvertes.fr/hal-01884119

M. Breton and L. M. Mir, Microsecond and nanosecond electric pulses in cancer treatments, Bioelectromagnetics, vol.33, pp.106-123, 2012.

. Brown, Development of an adaptive electroporation system for intratumoral plasmid DNA delivery. spheroid model, International Journal of Pharmacognosy, vol.423, pp.7-15, 2012.

. Clausen, Coplanar Electrode Layout Optimized for Increased Sensitivity for, Electrical Impedance Spectroscopy. Micromachines, vol.6, pp.110-120, 2014.

J. Cottet, HEK cell aggregation in flow conditions using 3 electrodes in a 4 electrode configuration design, 2018.

J. Cottet, HEK cell aggregation in flow conditions using 3 electrodes in a 8 electrode configuration design, 2018.

. Cottet, MyDEP: A New Computational Tool for Dielectric Modeling of Particles and Cells, Biophysical Journal, vol.116, pp.12-18, 2019.
URL : https://hal.archives-ouvertes.fr/hal-01884125

. Cottet, MyDEP: a new computational tool for dielectric modeling of particles and cells, 2018.
URL : https://hal.archives-ouvertes.fr/hal-01884125

. Cottet, Dielectrophoresis-assisted creation of cell aggregates under flow conditions using planar electrodes, Electrophoresis, vol.40, pp.1498-1509, 2019.
URL : https://hal.archives-ouvertes.fr/hal-02052550

. Cottet, How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study, Microfluidics and Nanofluidics, vol.23, 2019.
URL : https://hal.archives-ouvertes.fr/hal-01970310

. Cottet, A reproducible method for µm precision alignment of PDMS microchannels with on-chip electrodes using a mask aligner, Biomicrofluidics, vol.11, p.64111, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01884118

W. H. Coulter, Means for counting particles suspended in a fluid, 1953.

W. H. Coulter and W. R. Hogg, Signal modulated apparatus for generating and detecting resistive and reactive changes in a modulated current path for particle classification and analysis, 1970.

W. H. Coulter and C. M. Rodriguez, Particle analyzer for measuring the resistance and reactance of a particle, 1988.

E. B. Cummings and A. K. Singh, Dielectrophoresis in microchips containing arrays of insulating posts: Theoretical and experimental results, Analytical Chemistry, vol.75, pp.4724-4731, 2003.

A. B. Dababneh and I. T. Ozbolat, Bioprinting Technology: A Current State-of-the-Art Review, Journal of Manufacturing Science and Engineering-Transactions of the Asme, vol.136, 2014.

D. Ninno, Coplanar electrode microfluidic chip enabling accurate sheathless impedance cytometry, Lab on a Chip, vol.17, pp.1158-1166, 2017.

. Wagenaar, Towards microfluidic sperm refinement: impedance-based analysis and sorting of sperm cells, Lab on a Chip, vol.16, pp.1514-1522, 2016.

N. Demierre, Continuous-flow separation of cells in a lab-on-a-chip using "liquid electrodes" and multiple-frequency dielectrophoresis, EPFL, 2008.

. (demierre, Characterization and optimization of liquid electrodes for lateral dielectrophoresis, Lab on a Chip, vol.7, pp.355-365, 2007.

. (demierre, Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis, Sensors and Actuators B-Chemical, vol.132, pp.388-396, 2008.

I. Doh and Y. H. Cho, A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process, Sensors and Actuators aPhysical, vol.121, pp.59-65, 2005.

. Dona, Functional in vivo gene transfer into the myofibers of adult skeletal muscle, Biochemical and Biophysical Research Communications, vol.312, pp.1132-1138, 2003.

. Dontu, In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells, Genes & Development, vol.17, pp.1253-1270, 2003.

. Erdem, A numerical approach for dielectrophoretic characterization and separation of human hematopoietic cells, International Journal of Engineering Research & Technology (IJERT), vol.6, pp.1079-1082, 2017.

J. M. Escoffre, M. P. Rols, and . Electrochemotherapy, Current Pharmaceutical Design, vol.18, pp.3406-3415, 2012.

. Evander, Microfluidic impedance cytometer for platelet analysis, Lab on a Chip, vol.13, pp.722-729, 2013.

. Fennema, Spheroid culture as a tool for creating 3D complex tissues, Trends in Biotechnology, vol.31, pp.108-115, 2013.

. Ferrari, Biological samples micromanipulation by means of optical tweezers, pp.575-581, 2005.

. Fiedler, Dielectrophoretic sorting of particles and cells in a microsystem, Analytical Chemistry, vol.70, 1909.

K. R. Foster and H. P. Schwan, Dielectric properties of tissues and biological materials: a critical review, Critical Reviews in Biomedical Engineering, vol.17, pp.25-104, 1989.

. Fox, Electroporation of cells in microfluidic devices: A review, Analytical and Bioanalytical Chemistry, vol.385, pp.474-485, 2006.

. Frénéa, Positioning living cells on a high-density electrode array by negative dielectrophoresis, Materials Science and Engineering: C, vol.23, pp.597-603, 2003.

. Fuhr, Levitation, Holding, and Rotation of Cells within Traps Made by High-Frequency Fields, Biochimica et Biophysica Acta, vol.1108, pp.215-223, 1992.

. Fuhr, Cell manipulation and cultivation under a.c. electric field influence in highly conductive culture media, Biochimica et Biophysica Acta (BBA) -Reviews on Cancer, vol.1201, pp.353-360, 1994.

. Fuhr, Asynchronous Traveling-Wave Induced Linear Motion of Living Cells, Studia Biophysica, vol.140, pp.79-102, 1991.

Z. R. Gagnon, Cellular dielectrophoresis: applications to the characterization, manipulation, separation and patterning of cells, Electrophoresis, vol.32, pp.2466-2487, 2011.

P. R. Gascoyne and S. Shim, Isolation of circulating tumor cells by dielectrophoresis, Cancers (Basel), vol.6, pp.545-579, 2014.

. Gascoyne, NumericalAnalysis of the Influence of Experimental Conditions on the Accuracy of Dielectric Parameters Derived from Electrorotation Measurements, Bioelectrochemistry and Bioenergetics, vol.36, pp.115-125, 1995.

. Gascoyne, Dielectrophoretic Separation of Mammalian-Cells Studied by Computerized Image-Analysis, Measurement Science and Technology, vol.3, pp.439-445, 1992.

. Gawad, Dielectric spectroscopy in a micromachined flow cytometer: theoretical and practical considerations, Lab on a Chip, vol.4, pp.241-251, 2004.

. Gawad, Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing, Lab on a Chip, vol.1, pp.76-82, 2001.

E. Gersing, Impedance spectroscopy on living tissue for determination of the state of organs, Bioelectrochemistry and Bioenergetics, vol.45, pp.145-149, 1998.

. Gil-cardeza, Suicide gene therapy on spontaneous canine melanoma: correlations between in vivo tumors and their derived multicell spheroids in vitro, Gene Therapy, vol.17, pp.26-36, 2010.

H. Glasser and G. Fuhr, Cultivation of cells under strong ac-electric field-differentiation between heating and trans-membrane potential effects, Bioelectrochemistry and Bioenergetics, vol.47, pp.301-310, 1998.

. Golberg, Tissue heterogeneity in structure and conductivity contribute to cell survival during irreversible electroporation ablation by "electric field sinks, Scientific Reports, vol.5, 2015.

. Gorges, Accession of Tumor Heterogeneity by Multiplex Transcriptome Profiling of Single Circulating Tumor Cells, Clinical Chemistry, vol.62, pp.1504-1515, 2016.

. Gray, Engineering amount of cell-cell contact demonstrates biphasic proliferative regulation through RhoA and the actin cytoskeleton, Experimental Cell Research, vol.314, pp.2846-2854, 2008.

, Dielectrophoretic registration of living cells to a microelectrode array, 2004.

. Han, Microfluidic Lysis of Human Blood for Leukocyte Analysis Using Single Cell Impedance Cytometry, Analytical Chemistry, vol.84, pp.1070-1075, 2012.

T. Hanai, Electrical properties of emulsions. in Emulsion science, pp.353-478, 1968.

T. Hanai, Theory of the dielectric dispersion due to the interfacial polarization and its application to emulsions, Kolloid-Zeitschrift, vol.171, pp.23-31, 1960.

. Hanai, Dielectric theory of concentrated suspension of shell-spheres in particular reference to the analysis of biological cell suspensions, Bulletin of the Institute for Chemical Research, Kyoto University, vol.57, pp.297-305, 1979.

. Hanai, Analysis of Dielectric Relaxations of W/O Emulsions in the Light of Theories of Interfacial Polarization, Colloid and Polymer Science, vol.260, pp.1029-1034, 1982.

H. S. Hatfield, E. Hatschek, and P. C. Thorne, Metal sols in nondissociating liquids. I. Nickel in toluene and benzene, Proceedings of the Royal Society of London Series a-Containing Papers of a Mathematical and Physical Character, vol.103, pp.276-284, 1923.

. Heath, Single-cell analysis tools for drug discovery and development, Nature Reviews Drug Discovery, vol.15, pp.204-216, 2016.

. Heida, Dielectrophoretic trapping of dissociated fetal cortical rat neurons, IEEE Transactions on Biomedical Engineering, vol.48, pp.921-930, 2001.

. Henslee, Selective concentration of human cancer cells using contactless dielectrophoresis, Electrophoresis, vol.32, pp.2523-2529, 2011.

. Holcomb, Electrode array detector for microchip capillary electrophoresis, Analyst, vol.134, pp.486-492, 2009.

M. ;. Holmes, D. Morgan, and H. , Single Cell Impedance Cytometry for Identification and Counting of CD4 T-Cells in Human Blood Using Impedance Labels, Analytical Chemistry, vol.82, pp.1455-1461, 2010.

. Holmes, Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry, Lab on a Chip, vol.9, pp.2881-2889, 2009.

R. Hooke and . Micrographia, Some physiological descriptions of minute bodies made by magnifying glasses :with observations and inquiries thereupon, 1665.

. Hsiao, Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates, 2012.

, Biomedical Microdevices, vol.14, pp.313-323, 2012.

. Huang, Introducing dielectrophoresis as a new force field for field-flow fractionation, Biophysical Journal, vol.73, pp.1118-1129, 1997.

M. P. Hughes, Fifty years of dielectrophoretic cell separation technology, Biomicrofluidics, vol.10, 2016.

M. P. Hughes, Nanoelectromechanics in engineering and biology, 2002.

. Huh, Microfabrication of human organs-on-chips, Nature Protocols, vol.8, pp.2135-2157, 2013.

. Huh, Reconstituting Organ-Level Lung Functions on a Chip. Science, vol.328, pp.1662-1668, 2010.

. Ibrahim, The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells, Microelectronic Engineering, vol.97, pp.369-374, 2012.

. Ingram, Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a NASA bioreactor, In Vitro Cellular & Developmental Biology-Animal, vol.33, pp.459-466, 1997.

. Irimajiri, Dielectric monitoring of rouleaux formation in human whole blood: a feasibility study, Biochimica et Biophysica Acta (BBA) -Reviews on Cancer, vol.1290, pp.207-209, 1996.

. Irimajiri, A dielectric theory of "multi-stratified shell" model with its application to a lymphoma cell, Journal of Theoretical Biology, vol.78, pp.251-269, 1979.

. Irimajiri, Dielectric modeling of biological cells. Models and algorithm, Bull. Inst. Chem. Res., Kyoto University, vol.69, pp.421-438, 1991.

. Jaeger, Contactfree single-cell cultivation by negative dielectrophoresis, Journal of Physics DApplied Physics, vol.41, p.175502, 2008.

. Johnstone, In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells, Experimental Cell Research, vol.238, pp.265-272, 1998.

T. B. Jones, Basic theory of dielectrophoresis and electrorotation, Electromechanics of Particles, vol.22, pp.33-42, 1995.

. Kakutani, Electrorotation of non-spherical cells: Theory for ellipsoidal cells with an arbitrary number of shells, Bioelectrochemistry and Bioenergetics, vol.31, issue.93, p.80002, 1993.

. Kelm, Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types, Biotechnology and Bioengineering, vol.83, pp.173-180, 2003.

. Kilchenmann, Metal-Coated SU-8 Structures for High-Density 3-D Microelectrode Arrays, Journal of Microelectromechanical Systems, vol.25, pp.425-431, 2016.

. Kim, Automatic aligning and bonding system of PDMS layer for the fabrication of 3D microfluidic channels, Sensors and Actuators a-Physical, vol.119, pp.593-598, 2005.

. Kim, Recent trends in microelectrode array technology for in vitro neural interface platform, Biomedical Engineering Letters, vol.4, pp.129-141, 2014.

. Kipper, Control and automation of multilayered integrated microfluidic device fabrication, Lab on a Chip, vol.17, pp.557-566, 2017.

. Kuttel, Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer, Acta Tropica, vol.102, pp.63-68, 2007.

M. A. Lancaster and J. A. Knoblich, Organogenesis in a dish: Modeling development and disease using organoid technologies, Science, vol.345, 2014.

. Land, Low cost fabrication and assembly process for re-usable 3D polydimethylsiloxane (PDMS) microfluidic networks, Biomicrofluidics, vol.5, pp.36502-365026, 2011.

. Lapizco-encinas, Dielectrophoretic concentration and separation of live and dead bacteria in an array of insulators, Analytical Chemistry, vol.76, pp.1571-1579, 2004.

S. W. Lee and S. S. Lee, Shrinkage ratio of PDMS and its alignment method for the wafer level process. Microsystem TechnologiesMicro-and Nanosystems-Information Storage and Processing Systems, vol.14, pp.205-208, 2008.

. Lei, Viscous torque on a sphere under arbitrary rotation, Applied Physics Letters, vol.89, 2006.

A. Lenshof and T. Laurell, Continuous separation of cells and particles in microfluidic systems, Chemical Society Reviews, vol.39, pp.1203-1217, 2010.

J. M. Levsky and R. H. Singer, Gene expression and the myth of the average cell, Trends in Cell Biology, vol.13, pp.2-8, 2003.

, Desktop aligner for fabrication of multilayer microfluidic devices, Eleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences -The proceedings of microTAS 2007 Conference, vol.86, p.75008, 2007.

Y. L. Li and K. V. Kaler, Dielectrophoretic fluidic cell fractionation system, Analytica Chimica Acta, vol.507, pp.151-161, 2004.

. Llorens-bobadilla, Single-Cell Transcriptomics Reveals a Population of Dormant Neural Stem Cells that Become Activated upon Brain Injury, Cell Stem Cell, vol.17, pp.329-340, 2015.

. Lobjois, Cell cycle and apoptotic effects of SAHA are regulated by the cellular microenvironment in HCT116 multicellular tumour spheroids, European Journal of Cancer, vol.45, pp.2402-2411, 2009.

. Lotters, The mechanical properties of the rubber elastic polymer polydimethylsiloxane for sensor applications, Journal of Micromechanics and Microengineering, vol.7, pp.145-147, 1997.

. Madsen, Multicell tumor spheroids in photodynamic therapy, Lasers in Surgery and Medicine, vol.38, pp.555-564, 2006.

. Manaresi, Improved on-chip impedimetric immuno-detection of subpopulations of cells toward single-cell resolution, Proceedings of the 28th European, vol.230, pp.825-831, 2002.

R. M. Manczak, Microfluidic device for quantification of subpopulations of cells, 2016.
URL : https://hal.archives-ouvertes.fr/tel-01315630

. Marcus, Microfluidic single-cell mRNA isolation and analysis, Analytical Chemistry, vol.78, pp.3084-3089, 2006.

. Markx, Field-flow fractionation using non-uniform electric fields, vol.20, pp.2857-2872, 1997.

. Markx, Separation of viable and non-viable yeast using dielectrophoresis, Journal of Biotechnology, vol.32, pp.29-37, 1994.

. Martinez-duarte, A novel approach to dielectrophoresis using carbon electrodes, Electrophoresis, vol.32, pp.2385-2392, 2011.

. Marty, Electrochemotherapy -An easy, highly effective and safe treatment of cutaneous and subcutaneous metastases, 2006.
URL : https://hal.archives-ouvertes.fr/hal-00093693

). Electrochemotherapy and . Study, European Journal of Cancer Supplements, vol.4, pp.3-13, 2006.

U. Marx and V. Sandig, Drug Testing in vitro, 2006.

, Method and apparatus for the manipulation of particles by means of dielectrophoresis, 2000.

. Medoro, A lab-on-a-chip for cell detection and manipulation, IEEE Sensors Journal, vol.3, pp.317-325, 2003.

R. Meissner, Impedance spectroscopy for in vitro toxicology, EPFL, 2013.

. Mellor, Optimising non-viral gene delivery in a tumour spheroid model, Journal of Gene Medicine, vol.8, pp.1160-1170, 2006.

. Menad, From bipolar to quadrupolar electrode structures: an application of bond-detach lithography for dielectrophoretic particle assembly, Langmuir, vol.30, pp.5686-5693, 2014.
URL : https://hal.archives-ouvertes.fr/hal-00988569

. Menad, nDEP-driven cell patterning and bottom-up construction of cell aggregates using a new bioelectronic chip, Acta Biomaterialia, vol.17, pp.107-114, 2015.
URL : https://hal.archives-ouvertes.fr/hal-01113992

. Mir, Standard operating procedures of the electrochemotherapy: Instructions for the use of bleomycin or cisplatin administered either systemically or locally and electric pulses delivered by the CliniporatorTM by means of invasive or non-invasive electrodes, European Journal of Cancer Supplements, vol.4, pp.14-25, 2006.

. Mironov, Organ printing: computer-aided jet-based 3D tissue engineering, Trends in Biotechnology, vol.21, pp.157-161, 2003.

. Mittal, NDEP microwells for single-cell patterning in physiological media, Lab on a Chip, vol.7, pp.1146-1153, 2007.

. Moraes, Solving the shrinkageinduced PDMS alignment registration issue in multilayer soft lithography, Journal of Micromechanics and Microengineering, vol.19, 2009.

. Moreira, Fabrication of a multichannel PDMS/glass analytical microsystem with integrated electrodes for amperometric detection, Lab on a Chip, vol.9, pp.115-121, 2009.

H. Morgan and N. G. Green, AC electrokinetics: colloids and nanoparticles, 2003.

H. Spencer-;-morgan and D. Spencer, Microfluidic Impedance Cytometry for Blood Cell Analysis, Microfluidics for Medical, 2015.

. Morgan, Single cell dielectric spectroscopy, Journal of Physics D: Applied Physics, vol.40, pp.61-70, 2007.

. Moroni, Integrating novel technologies to fabricate smart scaffolds, Journal of Biomaterials Science, vol.19, pp.543-572, 2008.

. Nakamura, Apoptosis induction of human lung cancer cell line in multicellular heterospheroids with humanized antiganglioside GM2 monoclonal antibody, Cancer Research, vol.59, pp.5323-5330, 1999.

. Napolitano, Dynamics of the self-assembly of complex cellular aggregates on micromolded nonadhesive hydrogels, Tissue Engineering, vol.13, pp.2087-2094, 2007.

. Napolitano, Scaffold-free threedimensional cell culture utilizing micromolded nonadhesive hydrogels, BioTechniques, vol.43, pp.496-500, 2007.

. Narayanamurthy, Microfluidic hydrodynamic trapping for single cell analysis: mechanisms, methods and applications, Analytical Methods, vol.9, pp.3751-3772, 2017.

. Neumann, Transfer into Mouse Lyoma Cells by Electroporation in High Electric-Fields, vol.1, pp.841-845, 1982.

. Nieminen, Physics of optical tweezers. Laser Manipulation of Cells and Tissues, vol.82, pp.207-236, 2007.

. Pampaloni, The third dimension bridges the gap between cell culture and live tissue, Nature Reviews Molecular Cell Biology, vol.8, pp.839-845, 2007.

G. Pesch, On the Dielectrophoretic Particle Retention in Porous Media, 2018.

. Petchakup, Advances in Single Cell Impedance Cytometry for Biomedical Applications. Micromachines, vol.8, 2017.

R. Pethig, Review article-dielectrophoresis: status of the theory, technology, and applications, Biomicrofluidics, vol.4, p.22811, 2010.

R. R. Pethig, Dielectrophoresis: Theory, methodology and biological applications, 2017.

H. A. Pohl, Dielectrophoresis : the behavior of neutral matter in nonuniform electric fields, 1978.

H. A. Pohl, The Motion and Precipitation of Suspensoids in Divergent Electric Fields, Journal of Applied Physics, vol.22, pp.869-871, 1951.

. Pontani, Biomimetic emulsions reveal the effect of mechanical forces on cell-cell adhesion, Proc Natl Acad Sci U S A, vol.109, pp.9839-9844, 2012.

. Punniyakoti, Hydrogen Silsesquioxane-Based Nanofluidics, Advanced Materials Interfaces, vol.4, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01701361

. Puttaswamy, Enhanced cell viability and cell adhesion using low conductivity medium for negative dielectrophoretic cell patterning, Biotechnol J, vol.5, pp.1005-1015, 2010.

. Qian, Integrated planar concentric ring dielectrophoretic (DEP) levitator, Journal of Electrostatics, vol.55, pp.65-79, 2002.

V. Raicu and Y. Feldman, Dielectric Relaxation in Biological Systems: Physical Principles, Methods, and Applications, 2015.

. Raicu, Dielectric properties of rat liver in vivo: analysis by modeling hepatocytes in the tissue architecture, Bioelectrochemistry and Bioenergetics, vol.47, pp.333-342, 1998.

. Ramos, Ac electrokinetics: a review of forces in microelectrode structures, Journal of Physics D-Applied Physics, vol.31, pp.2338-2353, 1998.

. Reale, Electrical measurement of cross-sectional position of particles flowing through a microchannel, Microfluidics and Nanofluidics, vol.22, 2018.

. (reichle, Electrorotation in octopole micro cages, Journal of Physics D-Applied Physics, vol.32, pp.2128-2135, 1999.

. (reichle, A new microsystem for automated electrorotation measurements using laser tweezers, Biochimica Et Biophysica Acta-Bioenergetics, vol.1459, pp.218-229, 2000.

. (ren, A Simple and Reliable PDMS and SU-8 Irreversible Bonding Method and Its Application on a Microfluidic-MEA Device for, Neuroscience Research. Micromachines, vol.6, 1923.

. Rivron, Tissue assembly and organization: Developmental mechanisms in microfabricated tissues, Biomaterials, vol.30, pp.4851-4858, 2009.

. Rodriguez-devora, Direct assembling methodologies for high-throughput bioscreening, Biotechnol J, vol.6, pp.1454-1465, 2011.

. Rols, In vivo electrically mediated protein and gene transfer in murine melanoma, Nature Biotechnology, vol.16, pp.168-171, 1998.

. Ross, Temperature Measurement in Microfluidic Systems Using a Temperature-Dependent Fluorescent Dye, Analytical Chemistry, vol.73, pp.4117-4123, 2001.

A. J. Sale and W. A. Hamilton, Effects of high electric fields on micro-organisms. 3. Lysis of erythrocytes and protoplasts, Biochimica et Biophysica Acta (BBA) -Reviews on Cancer, vol.163, issue.68, pp.90030-90038, 1968.

J. Sambrook and D. W. Russell, DNA transfection by electroporation, CSH Protoc, 2006.

. Santini, Apoptosis, cell adhesion and the extracellular matrix in the three-dimensional growth of multicellular tumor spheroids, Critical Reviews in Oncology Hematology, vol.36, pp.75-87, 2000.

. Schaefer, The complex dielectric spectrum of heart tissue during ischemia, Bioelectrochemistry, vol.58, pp.171-180, 2002.

H. P. Schwan, Electrical Properties of Tissue and Cell Suspensions, Advances in Biological and Medical Physics, vol.5, pp.147-209, 1957.

H. Schwan and . Sebastian, Formation of multilayer aggregates of mammalian cells by dielectrophoresis, Proceedings of 16th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. A70-A71, vol.71, pp.1769-1777, 1994.

. Sebastian, Tissue engineering with electric fields: immobilization of mammalian cells in multilayer aggregates using dielectrophoresis, Biotechnology and Bioengineering, vol.98, pp.694-700, 2007.

. Seger-sauli, Temperature measurements in microfluidic systems: heat dissipation of negative dielectrophoresis barriers, Electrophoresis, vol.26, pp.2239-2246, 2005.

. Shaker, An impedance-based flow microcytometer for single cell morphology discrimination, Lab on a Chip, vol.14, pp.2548-2555, 2014.

. Shi, Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW), Lab on a Chip, vol.9, pp.3354-3359, 2009.

. Shim, Antibody-independent isolation of circulating tumor cells by continuous-flow dielectrophoresis, Biomicrofluidics, vol.7, p.11807, 2013.

A. H. Sihvola and J. A. Kong, Effective permittivity of dielectric mixtures, IEEE Transactions on Geoscience and Remote Sensing, vol.26, pp.420-429, 1988.

. Sivakumarasamy, A simple and inexpensive technique for PDMS/silicon chip alignment with sub-?m precision, Anal. Methods, vol.6, pp.97-101, 2014.

. Sivaraman, A microscale in vitro physiological model of the liver: Predictive screens for drug metabolism and enzyme induction, Current Drug Metabolism, vol.6, pp.569-591, 2005.

. Slaughter, Hydrogels in regenerative medicine, Advanced Materials, vol.21, pp.3307-3329, 2009.

M. Lai and E. , Identification of mesenchymal stem cell differentiation state using dual-micropore microfluidic impedance flow cytometry, Analytical Methods, vol.8, pp.7437-7444, 2016.

. Song, A microfluidic impedance flow cytometer for identification of differentiation state of stem cells, Lab on a Chip, vol.13, pp.2300-2310, 2013.

. Souza, Three-dimensional tissue culture based on magnetic cell levitation, Nature Nanotechnology, vol.5, pp.291-296, 2010.

. Spencer, Microfluidic impedance cytometry of tumour cells in blood, Biomicrofluidics, vol.8, 2014.

D. Spencer and H. Morgan, Positional dependence of particles in microfludic impedance cytometry, Lab on a Chip, vol.11, pp.1234-1239, 2011.

J. Suehiro and R. Pethig, The dielectrophoretic movement and positioning of a biological cell using a three-dimensional grid electrode system, Journal of Physics D: Applied Physics, vol.31, pp.3298-3305, 1998.

M. Sun, T. Morgan, and H. , Single-cell microfluidic impedance cytometry: a review, Microfluidics and Nanofluidics, vol.8, pp.423-443, 2010.

R. M. Sutherland, Cell and Environment Interactions in Tumor Microregions -the Multicell Spheroid Model, Science, vol.240, pp.177-184, 1988.

. Sutherland, Growth of multicell spheroids in tissue culture as a model of nodular carcinomas, Journal of the National Cancer Institute, vol.46, pp.113-120, 1971.

B. M. Taff and J. Voldman, A scalable addressable positivedielectrophoretic cell-sorting array, Analytical Chemistry, vol.77, pp.7976-7983, 2005.

. Talary, Electromanipulation and separation of cells using travelling electric fields, Journal of Physics D-Applied Physics, vol.29, pp.2198-2203, 1996.

. Tan, Cells lying on a bed of microneedles: an approach to isolate mechanical force, Proc Natl Acad Sci U S A, vol.100, pp.1484-1489, 2003.

. Torisawa, Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test, Biomaterials, vol.26, pp.2165-2172, 2005.

. Trainito, The Electrorotation as a Tool to Monitor the Dielectric Properties of Spheroid During the Permeabilization, Journal of Membrane Biology, vol.249, pp.593-600, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01284729

T. Y. Tsong, Molecular Recognition and Processing of Periodic Signals in Cells -Study of Activation of Membrane Atpases by Alternating ElectricFields, Biochimica et Biophysica Acta, vol.1113, pp.90034-90042, 1992.

. Tung, High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array, Analyst, vol.136, pp.473-478, 2011.

. Valero, Gene transfer and protein dynamics in stem cells using single cell electroporation in a microfluidic device, Lab on a Chip, vol.8, pp.62-67, 2008.

J. Voldman, Dielectrophoretic traps for cell manipulation, BioMEMS and Biomedical Nanotechnology, vol.4, pp.159-186, 2007.

J. Voldman, Electrical forces for microscale cell manipulation, Annual Review of Biomedical Engineering, vol.8, pp.425-454, 2006.

. Voldman, Holding forces of single-particle dielectrophoretic traps, Biophysical Journal, vol.80, pp.531-541, 2001.

. Voldman, A Microfabrication-Based Dynamic Array Cytometer, Analytical Chemistry, vol.74, pp.3984-3990, 2002.

D. Bodovitz-;-wang and S. Bodovitz, Single cell analysis: the new frontier in 'omics', Trends in Biotechnology, vol.28, pp.281-290, 2010.

. Wang, Dielectrophoresis switching with vertical sidewall electrodes for microfluidic flow cytometry, Lab on a Chip, vol.7, 2007.

. Wang, Changes in Friend Murine Erythroleukemia CellMembranes during Induced-Differentiation Determined by Electrorotation, Biochimica Et Biophysica Acta-Biomembranes, vol.1193, pp.330-344, 1994.

M. Washizu, Electrostatic Manipulation of Biological Objects, Journal of Electrostatics, vol.25, pp.109-123, 1990.

, Biological Effects Due to Weak Electric and Magnetic Fields: The Temperature Variation Threshold, Biophysical Journal, vol.76, pp.77455-77457, 1999.

. Ye, Research on the cast molding process for high quality PDMS molds, Microelectronic Engineering, vol.86, pp.310-313, 2009.

. Zanoni, 3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained, Sci Rep, vol.6, 2016.

. Zhang, Sealing SU-8 microfluidic channels using PDMS, Biomicrofluidics, vol.5, 2011.

. Zhao, Single-Cell Electrical Phenotyping Enabling the Classification of Mouse Tumor Samples, Sci Rep, vol.6, 2016.

. Zhao, Electrical property characterization of neural stem cells in differentiation, PLoS ONE, vol.11, 2016.

. Zhao, Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity, Biosensors and Bioelectronics, vol.57, pp.245-253, 2014.

. Zhong, A microfluidic processor for gene expression profiling of single human embryonic stem cells, Lab on a Chip, vol.8, pp.68-74, 2008.

. Zhou, Recent developments in PDMS surface modification for microfluidic devices, Electrophoresis, vol.31, pp.2-16, 2010.

. Zhu, A Versatile Bonding Method for PDMS and SU-8 and Its Application towards a, Multifunctional Microfluidic Device. Micromachines, vol.7, 2016.

. Zimmermann, A combined patch-clamp and electrorotation study of the voltage-and frequencydependent membrane capacitance caused by structurally dissimilar lipophilic anions, Journal of Membrane Biology, vol.221, pp.107-121, 2008.

J. Cottet, C. Vaillier, F. Buret, M. Frenea-robin, and P. Renaud, A reproducible method for µm precision alignment of PDMS microchannels with on-chip electrodes using a mask aligner, Biomicrofluidics, vol.11, p.64111, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01884118

B. Brazey, J. Cottet, A. Bolopion, H. Van-lintel, P. Renaud et al., Impedance-based real-time position sensor for lab-on-a-chip devices, Lab on a Chip, vol.18, pp.818-831, 2018.
URL : https://hal.archives-ouvertes.fr/hal-01884119

J. Cottet, O. Fabregue, C. Berger, F. Buret, P. Renaud et al., A New Computational Tool for Dielectric Modeling of Particles and Cells
URL : https://hal.archives-ouvertes.fr/hal-01884125

, Biophysical Journal, vol.116, pp.12-18, 2019.

J. Cottet, A. Kehren, H. Van-lintel, F. Buret, M. Frénéa-robin et al., How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study, Microfluidics and Nanofluidics, vol.23, 2019.
URL : https://hal.archives-ouvertes.fr/hal-01970310

J. Cottet, A. Kehren, S. Lasli, H. Van-lintel, and F. Buret,

P. Renaud, Dielectrophoresis-assisted creation of cell aggregates under flow conditions using planar electrodes, Electrophoresis, vol.40, pp.1498-1509, 2019.
URL : https://hal.archives-ouvertes.fr/hal-02052550

J. Cottet, O. Fabregue, C. Berger, F. Buret, P. Renaud et al., MyDEP: a new computational tool for dielectric modeling of particles and cells, 2018.
URL : https://hal.archives-ouvertes.fr/hal-01884125

F. Buret, J. Cottet, M. Frénéa-robin, J. Marchalot, S. Menad et al., Dielectrophoretic formation of cell aggregates mimicking tumors for in vitro study of electrochemotherapy in, 10th International Symposium on Electric and Magnetic Fields, pp.12-14, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01393391

J. Cottet, M. Frénéa-robin, F. Buret, and P. Renaud, Microfluidic chip for the creation and characterization of cell aggregates with electrical engineering techniques in GDR Micro et Nanofluidique (IPGG, Juin, pp.23-24, 2016.

J. Cottet, M. Frénéa-robin, F. Buret, and P. Renaud, Creation of cell aggregates by dielectrophoresis for the in vitro study of electrochemotherapy in ElectroporationBased Technologies and Treatments, pp.13-19, 2016.

A. Seth, D. Voyer, J. Cottet, J. Marchalot, M. Frénéa-robin et al., Modélisation de la dynamique de l'électroporation à l'échelle tissulaire in Modéliser le vivant, Journée scientifique de la Faculté des Sciences et Technologies, UCBL, vol.22, 2017.

J. Cottet, A. Kehren, H. Van-lintel, F. Buret, M. Frénéa-robin et al., Improvement of the sensitivity of a coplanar electrode layout for Electrical Impedance Spectroscopy in NanoBioTech-Montreux, 2017.

J. Cottet, O. Fabrègue, J. Marchalot, R. Scorretti, F. Buret et al.,

P. Renaud, Bottom-up assembly of cells in flow with dielectrophoresis in GDR MicroNanoFluidique, pp.15-16, 2018.

J. Cottet, A. Kehren, H. Van-lintel, F. Buret, M. Frénéa-robin et al., Towards more sensitive coplanar electrode designs for analysis of single cells and small cell aggregates by Electrical Impedance Spectroscopy, 2nd International Conference of Microfluidics, 2018.
URL : https://hal.archives-ouvertes.fr/hal-01884145

J. Cottet, A. Kehren, H. Van-lintel, F. Buret, M. Frénéa-robin et al., Creation of cell aggregates under flow with dielectrophoresis using planar electrodes in Biosensors, 2018.

J. Cottet, O. Fabrègue, J. Marchalot, R. Scorretti, F. Buret et al., Développement de microsystèmes utilisant des champs électriques 238

, pour la création et la caractérisation d'agrégats de cellules in Symposium de Génie Electrique (SGE 2018), pp.3-5, 2018.

J. Cottet, O. Fabrègue, C. Berger, F. Buret, P. Renaud et al.,

, MyDEP, a software for dielectric modeling of particles and cells for simulation of dielectrophoretic response spectra in Dielectrophoresis, 2018.

. Kingdom, Juillet, pp.23-25, 2018.

J. Cottet, A. Kehren, S. Lasli, H. Van-lintel, and F. Buret,

P. Renaud, Cell trapping and aggregation in flow with dielectrophoresis using planar electrodes in Dielectrophoresis, pp.23-25, 2018.
URL : https://hal.archives-ouvertes.fr/hal-01946305

J. Cottet, A. Kehren, S. Lasli, H. Van-lintel, and F. Buret,

P. Renaud, 28-30 Novembre) (Poster) Grant and Prizes International mobility grants from the PALSE (2017) and iMUST LABEX (2018) Presentation prize "Early Career Researcher Award, 2018.