Manipulation of the illumination of an Adaptive Optics Flood Illumination Ophthalmoscope for functional imaging of the retina in-vivo

Abstract : As the only transparent optical window of our body, the eye gives a unique access to the observation of neural and vascular networks. Today however, a new era is opening up for high-resolution imaging, which should no longer be limited to giving access to tissue structures, but may also tackle their functions. In fact, biomarkers for the functioning of the whole human body can be found in retinal imaging. Neurodegenerative diseases (Parkinson's, Alzheimer's) or arterial hypertension could thus be diagnosed early by high precision imaging of the retina. Adaptive optics, applied to retinal imaging as early as 1997, significantly improved the spatial resolution of retinal images, leading to many retinal studies by ophthalmoscopy. In particular, it was coupled with the scanning ophthalmoscope, which became the most popular choice for its superior spatial resolution and in optical sectioning compared to full-field. However, unlike scanning systems, the full-field ophthalmoscope produces wide-field images with a high rate of acquisition and without distortion. In my thesis work, I intended to show how such a system, associated to imaging modalities adjusting geometrical settings of the illumination, could contribute to research on the retina. To achieve this ambitious goal, we modified the full-field ophthalmoscope built at the National Hospital of Quinze-Vingts with a specific image processing and two new instruments inspired by full-field microscopy. We have integrated these instruments into the illumination path of the ophthalmoscope to manipulate the geometry of the retinal illumination. These new implementations allow us to make use of more advanced imaging techniques, such as dark field imaging or noninvasive near infrared angiography. These imaging modalities have been exploited to image the retina functionally. We focused mainly on the light coupling function of photoreceptors and on blood perfusion.
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Submitted on : Friday, June 28, 2019 - 2:28:07 PM
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Elena Salas. Manipulation of the illumination of an Adaptive Optics Flood Illumination Ophthalmoscope for functional imaging of the retina in-vivo. Optics / Photonic. Sorbonne Université, 2019. English. ⟨tel-02168160⟩

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