Fonctionnalisation optimisée de différentes surfaces par des paires de FRET pour des applications de biodétection en plasmonique et en microfluidique

Abstract : Patient care quality relies on the availability of efficient diagnostics tools. Development of biosensors based on Förster resonance energy transfer (FRET) allows for the detection of specific biomarkers with high precision and sensitivity. FRET is a distance dependent energy transfer process that is frequently used in biosensing applications, in which biological recognition, functions or structures are within the 1 to 20 nm length scale. This PhD thesis presents the establishment of fabrication and functionalization processes for the optical FRET detection of molecules of biological interest, toward an application in clinical diagnostics and DNA sequencing.This work presents a FRET study on functionalized aluminum surfaces, which is the first step towards the development of a sequencing platform using plasmonics enhanced FRET. Quantitative FRET detection on silanized aluminum surfaces was extensively investigated and the results of different characterization methods (contact angle, FTIR spectroscopy, fluorescence imaging) are discussed in details.This manuscript also describes the development of a functionalized microfluidic device for the realization of a multiplexed FRET immunoassay. With the aim of designing a functionalized integrated device for FRET detection in microfluidic conditions, I developed a strategy for the realization of a microfluidic optical multiplexed FRET biosensor. Preliminary FRET results between two labeled antibodies in a microfluidic channel demonstrate the feasibility of such a biosensing platform.
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Alexandra Petreto. Fonctionnalisation optimisée de différentes surfaces par des paires de FRET pour des applications de biodétection en plasmonique et en microfluidique. Biophysique [physics.bio-ph]. Université Paris-Saclay, 2019. Français. ⟨NNT : 2019SACLS048⟩. ⟨tel-02165933⟩

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