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. Sm/chol and . Luvs, Continuous lines heating protocol and dotted lines cooling protocol, Means ± SEM of 4 and 7 independent experiments for PC and 5 and 8 for SM/Chol

, Fluorescence anisotropy (r) of py-met-chol. LUVs were incubated at (A) 15°C, (B) 35°C and (C) 50°C. Py-met-chol at 3.6%, PC LUVs in black and SM/Chol LUVs in red. Curves are the mean ± SEM of four independent experiments

, Ratios of Py-met-chol fluorescence wavelengths in function of temperature. The probe concentration in LUVs is 3.6%. (A), Ld/iso during heating and cooling

, Lo/Ld during heating and cooling. (E) exci/iso during heating. (F) isci/iso during cooling, Black lines (?,?) PC LUVs, red lines (?,?) SM/Chol LUVs and green lines (?,?) PC/SM/Chol LUVs. Continuous lines heating protocol and dotted lines cooling protocol

, Means ± SEM of 7 independent experiments for PC, 8 for SM/Chol and 6 for PC/SM/Chol. Annexes Supplementary material

S. Fig, Mean spectra of LUV PC and SM/Chol at different temperatures after cooling and heating. (A) spectra at 10°C, (B) at 35°C and (C) at 55°C. Red lines PC LUVs, black lines SM/Chol LUVs. Continuous lines from heating protocol and dotted lines from cooling protocol, Each line is the mean of 7 (PC LUVs) and 8 (SM/Chol LUVs) spectra from independent experiments

S. Fig, Subtraction of Ld-Lo Py-met-chol spectra (3.6%) at different temperatures. PC mean spectrum minus SM/Chol mean spectrum at (A) 10°C, (B) 35°C and (C) 55°C. Continuous lines for heating protocol and dotted line for cooling protocol

S. Fig, 474 nm excimer peak or multimer marker. Black lines PC LUVs, red lines SM/Chol LUVs. Continuous lines heating protocol and dotted lines cooling protocol, Evolution of Py-met-chol fluoescence wavelengths in function of temperature. The probe concentration in LUVs is 1.8%. (A)