Etude CLIMATOX : contribution à la caractérisation des bioaérosols d'habitats dégradés par les moisissures et à l'évaluation de leurs effets sur la santé.

Abstract : Air quality in houses is a major concern in Europe, as people spend most of their time indoors. According to the WHO, numerous houses are exposed to dampness that can lead to mold growth, causing health and economic damages.In this work, a global approach including both field study and laboratory experimentations was used to characterize the human exposure to bioaerosols in mold-damaged houses, to study their health effects on inhabitants and to propose a diagnostic process that could be applied to mold-damaged houses. We showed the recurrence of several fungal species, specifically Aspergillus versicolor, Penicillium chrysogenum and P. crustosum, which could be used as microbial indicators of airborne fungal contamination. In addition, our statistical analyses showed relations between the concentrations of recurrent molds in air and the levels of surface contamination by molds proposed by ANSES. Statistical link has also been found between mold exposure and respiratory or cutaneous symptoms described by the inhabitants. A cytotoxic potential evaluation of bioaerosols collected in these mold-damaged houses allowed to highlight a relation between the responses observed for the two cell lines (pulmonary A549 and cutaneous HaCaT).From a methodological point of view, qPCR coupled with flow cytometry appears to be a fast and reliable method that can be correlated to cultural approach, allowing to monitor the human exposure to Aspergillus versicolor in mold-damaged buildings. Eight recurrent fungal species identified in bioaerosols were also selected for a study in a climatic chamber that showed the effects of temperature and relative humidity on fungal growth and toxinogenic potential.
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Antoine Delanoe. Etude CLIMATOX : contribution à la caractérisation des bioaérosols d'habitats dégradés par les moisissures et à l'évaluation de leurs effets sur la santé.. Mycologie. Normandie Université, 2018. Français. ⟨NNT : 2018NORMC279⟩. ⟨tel-02163509⟩

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