Inhibition studies of metalloproteins by means of electrochemistry and spectroscopy

Abstract : Protein-ligand interaction studies help to better understand the structure and function of proteins. In the first part of the thesis cyt bd oxidase was studied. The protein from E. coli was successfully immobilised at gold nanoparticles modified electrodes. Thus, an electrochemical biosensor was created allowing testing some potential inhibitors of cyt bd from E. coli. The cyt bd from thermophilic G. thermodenitrificans was also studied. By means of IR, Raman spectroscopy and electrochemistry the protein was shown to be distinct from cyt bd from E. coli. A mutual influence of pH and temperature was demonstrated on the electrochemical and catalytical properties. The second part of the thesis focused on mitochondrial mitoNEET protein. The influence of the pH and various ligands was studied.
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Anton Nikolaev. Inhibition studies of metalloproteins by means of electrochemistry and spectroscopy. Other. Université de Strasbourg, 2018. English. ⟨NNT : 2018STRAF053⟩. ⟨tel-02146028⟩

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