Etude des interactions d'acides nucléiques avec des biomacromolécules et des nanoparticules : application à la vectorisation

Abstract : In this work, we were interested in the study, by optical spectroscopy, of the interactions that may have nucleic acids between them or with surfaces of various types such as proteins, or inorganic nanoparticles. In the first part, we present a structural study by UV and IR spectroscopy on nucleotide sequences contained in DsrA, a non-coding RNA. Several models of self-association are proposed. In the second part, the association of the Hfq protein with a duplex dA₂₀-dT₂₀ is studied by IR spectroscopy. This interaction occurs via a partial opening of the double-helix and a conformational transition of the sugars of the purine strand. In the third and fourth part, we are interested in the vectorization of oligonucleotides and peptides. Different strategies of maghemite nanoparticles surface functionalization are explored and the effectiveness of nano-vectors is assessed in vitro.
Keywords : Vectorization
Complete list of metadatas

Cited literature [73 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02144543
Contributor : Abes Star <>
Submitted on : Thursday, May 30, 2019 - 7:14:07 PM
Last modification on : Saturday, June 1, 2019 - 1:12:58 AM

File

edgalilee_th_2015_geinguenaud....
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02144543, version 1

Collections

Citation

Frédéric Geinguenaud. Etude des interactions d'acides nucléiques avec des biomacromolécules et des nanoparticules : application à la vectorisation. Chimie organique. Université Sorbonne Paris Cité, 2015. Français. ⟨NNT : 2015USPCD097⟩. ⟨tel-02144543⟩

Share

Metrics

Record views

131

Files downloads

87