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, Ajouter 1mL d'eau physiologique à 9 ?. Penser à ajouter un flacon contenant juste de l'eau physiologique pour faire un témoin solvant Bien fermer le bouchon et filmer avec du parafilm. Scotcher le flacon dans une coupelle, Protocoles d'extraction des MP Eau physiologique Placer 100mg d'échantillon de plastiques dans un flacon ambré de 4mL avec bouchon en téflon, vol.3

, Pepsine de porc Placer 100mg d'échantillon de plastiques dans un flacon ambré de 4mL avec bouchon en téflon

, Penser à ajouter un flacon contenant juste de la pepsine pour faire un témoin solvant Bien fermer le bouchon et filmer avec du parafilm. Scotcher le flacon dans une coupelle

, Ajouter 500µL de DMSO Penser à ajouter un flacon contenant juste du DMSO pour faire un témoin solvant Bien fermer le bouchon et filmer avec du parafilm. Scotcher le flacon dans une coupelle, DMSO Placer 50mg d'échantillon de plastiques dans un flacon ambré de 4mL avec bouchon en téflon

, Récupération de l'extrait

, Après l'incubation, récupérer les flacons et les centrifuger 5 minutes à 4000 rpm pour permettre la séparation des deux phases. Récupérer la phase liquide (environ 300µL) à l'aide d'une pointe de P200 extra longue et transférer par 100µL dans un flacon de 1,5mL. Conserver à l'abri de la lumière et à 4°C pendant quelques jours