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, Figure 1: Longitudinal sections of gilthead sea bream muscle at different post mortem times 6 h post mortem (Day 0) (a), 72 h (Day 3) (b), 96 h (Day 4) (c) and 144 h (Day 6) (d). A: A band
, Bars: a, b, c, d: 500 nm. Figure 5: Summary graphs of Rh123 fluorescence level at different states of respiration normalized to CCCP at different times of storage. The different times of storage were: Day 0, Day 1, Day 2, Day 3, and Day 4. Asterisks denote values which are significantly different at different times of storage. T-test was performed using SPSS, sar: sarcoplasm; sr: sarcoplasmic réticulum; Tr: triad; Z: Z line
, Les méthodes actuelles appliquées en routine pour déterminer la fraîcheur du poisson ne sont pas applicables à toutes les espèces et reflètent davantage un début d'altération du produit. Ainsi, la recherche d'indicateurs précoces de fraîcheur du poisson représente encore un défi majeur et d'actualité dans l
, Le but de ces travaux de thèse était de démontrer que les fonctions et l'intégrité mitochondriales étaient susceptibles de constituer des indicateurs précoces de la fraîcheur de filets de poisson
, Les fonctions et l'intégrité mitochondriales de cellules musculaires de filets de poisson ont été étudiées à différents temps de conservation post mortem à 4°C. Le modèle d'étude était la daurade royale (Sparus aurata) (lignée cellulaire de fibroblastes (SAF-1) et muscles de poisson)
, Dans un premier temps, la structure des
, La fonctionnalité des mitochondries a ensuite été évaluée selon deux approches : la respiration mitochondriale (oxygraphie) et le potentiel membranaire mitochondrial (?? m ) estimé avec la sonde fluorescente Rhodamine 123. À partir de 96 heures de conservation à 4°C (J4), ces deux paramètres ont été significativement impactés témoignant d'une altération des fonctions et de l'intégrité mitochondriales. Ces résultats sont ainsi en corrélation avec l'altération structurale observée par microscopie
, Mots clés : fraîcheur, daurade royale (Sparus aurata), mitochondrie, respiration mitochondriale, potentiel membranaire mitochondrial (?? m ), oxygraphie